摘要
该研究测量和统计了人工养殖四指马鲅(Eleutheronema tetradactylum)的形态及性别比例,并观察其性腺的组织学和形态学特征。结果显示,当盐度为10.8~13.0、水温为28.0~30.7℃时,在228尾7月龄四指马鲅样本中检测到雄性、雌性和雌雄同体3种个体,其比例分别为85.53%、5.70%和8.77%;3种个体的体质量、全长、体长和体高等形态性状无明显差异。解剖学观察显示,雌雄同体性腺的两背内侧为精巢,呈白色,两腹外侧为卵巢,呈浅黄偏红色。组织学显示精巢侧已有成熟精子形成,为典型精巢的Ⅳ或Ⅴ期特征,卵巢侧具卵巢腔,主要为卵原细胞和早期Ⅱ时相卵母细胞,为典型卵巢的Ⅱ期特征,可判断四指马鲅为雄性先熟雌雄同体;其性逆转过程可分为早、中、晚3个阶段,主要组织结构变化特征为卵巢结构逐渐形成并发育成Ⅱ期卵巢,而精巢组织则逐渐退化消失,在整个性逆转过程均检测到精巢的细胞凋亡信号。
In this study,we studied the morphological and sex ratio statistics of artificially cultured Eleutheronema tetradactylum,and observed their gonads by morphological and histological methods.The results show that when the salinity was 10.8–13.0 and the water temperature was 28.0–30.7℃,male,female and hermaphrodite accounted for 85.53%,5.70%and 8.77%,respectively in the 228 samples at age of 7 months.Besides,there was no significant difference in the body mass,total length,body length and body height among the three individuals.Anatomic observation shows that the two dorsolateral sides of androgynous gonads were white testis,and the two ventrolateral sides were ovaries which were yellowish and reddish.Histology shows that the mature sperm formed on the testis side,which is Stage IV or V feature of typical testis,and the ovary side had an ovarian cavity,mainly oocytes and early phase II oocytes,which were typical phase II characteristics of ovary.Thus,it can be concluded that E.tetradactylum is a protandrous hermaphrodite.The process of sex reversal can be divided into three stages:early,middle and late.The ovary structure gradually formed and developed into Stage II ovary,which is the main feature of tissue structure change.However,the testis structure gradually degenerated and disappeared.The apoptotic cell signal of the testis has been detected during the whole sex reversal process.
作者
蓝军南
区又君
温久福
李俊伟
牛莹月
李加儿
LAN Junnan;OU Youjun;WEN Jiufu;LI Junwei;NIU Yingyue;LI Jia'er(South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation&Utilization,Ministry of Agriculture and Rural Affairs,Guangzhou 510300,China;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources(Shanghai Ocean University),Ministry of Education/International Research Center for Marine Biosciences at Shanghai Ocean University,Ministry of Science and Technology/National Demonstration Center for Experimental Fisheries Science Education(Shanghai Ocean University),Shanghai 201306,China)
出处
《南方水产科学》
CAS
CSCD
北大核心
2020年第6期67-74,共8页
South China Fisheries Science
基金
国家重点研发计划“蓝色粮仓科技创新”重点专项(2018YFD0900200)
广东省现代农业产业技术体系(海水鱼产业)创新团队建设专项资金(2019KJ143)
中国水产科学研究院南海水产研究所中央级公益性科研院所基本业务费专项资金资助项目(2020TD55)
广东省省级乡村战略专项资金项目。
关键词
四指马鲅
雌雄同体
性逆转
组织学
细胞凋亡
Eleutheronema tetradactylum
Hermaphroditism
Sex reversal
Histology
Apoptosis
