摘要
为了探究外源施加脱落酸对干旱胁迫下谷子幼苗生长及生理特性的影响,以晋谷42号为研究对象,研究外源施加不同浓度(10-6 mol/L、10-5 mol/L、10-4 mol/L)的ABA对质量浓度为150 g/L的PEG-6000模拟干旱胁迫处理下的谷子幼苗的生长及生理指标变化.结果表明,与对照相比,模拟干旱处理降低了幼苗的相对含水量和叶绿素含量,提高了叶片中POD和SOD酶活性,同时增加了叶片中MDA含量和电导率.而与干旱胁迫相比,外源施加ABA处理提高了幼苗的相对含水量和叶绿素含量,进一步增强了POD和SOD酶活性,并且降低了幼苗MDA的含量和叶片电导率,减小了干旱胁迫对谷子幼苗的伤害.此外,研究发现,外源ABA对干旱胁迫下谷子幼苗抗旱性的提高作用存在浓度效应,其中10-5 mol/L ABA处理效果最佳,能最大程度提高谷子幼苗的抗旱能力.
In order to investigate the effect of exogenous abscisic acid(ABA)on the growth and physiological responses of millet seedlings under drought stress,Jingu No.42 was used as the research object to clarify that exogenous application of different concentrations(10-6 mol/L,10-5 mol/L,10-4 mol/L)of ABA on the changes of millet seedlings under 150 g/L PEG-6000 simulated drought stress.The results showed that compared with the control,the content of relative water and chlorophyll of the seedlings under the simulated drought treatment were reduced,whereas the activities of antioxidizes including peroxidase(POD)and superoxide dismutase(SOD)in the leaves were increased,same as the malondialdehyde(MDA)content and electrical conductivity in the leaves.Compared with seedlings under drought stress,the content of water and chlorophyll of the seedlings were significantly increased after exogenous ABA pretreatment,at the same time,the activities of POD and SOD were further enhanced.The MDA and leaf electrical conductivity in the seedlings under exogenous ABA pretreatment were decreased,thus reducing the injury to millet seedlings caused by drought stress.In addition,obvious gradient effect was represented during the application of exogenous ABA on these characteristics in improving the drought resistance of millet seedlings under simulated stress.In this study,the resistant responses represented by growth and physiological indexes in millet seedlings were most notable under 10-5 mol/L ABA treatment,which can most improve the drought resistance of millet seedlings.
作者
张笑
宋敏丽
ZHANG Xiao;SONG Minli(Department of Biology,Taiyuan Normal University,Jinzhong 030619,China)
出处
《太原师范学院学报（自然科学版）》
2020年第4期91-96,共6页
Journal of Taiyuan Normal University:Natural Science Edition
基金
山西省教育厅高等学校科技创新项目(2019L0802)。
关键词
干旱
脱落酸
谷子
生理特性
drought stress
abscisic acid
millet
physiological characteristic
