摘要
目的探讨爆震伤对大鼠神经功能的损伤作用及其初步机制。方法健康雄性SD大鼠32只,随机分为对照组及爆震伤组,爆震伤组接受TNT炸药爆炸制成爆震伤模型,对两组进行神经行为学评分,免疫荧光染色测定海马神经元凋亡,免疫组化法检测ZO-1蛋白的表达量,并比较两组的差异。结果爆震伤组大鼠神经行为学评分低于对照组,差异有统计学意义(P<0.05),且距离爆源越近,神经行为学评分越低。TUNEL检测显示爆震伤组神经元细胞凋亡率高于对照组,免疫组化结果显示爆震伤组ZO-1蛋白表达阳性率明显低于对照组,差异有统计学意义(P<0.05)。结论爆震伤对大鼠神经功能具有明显的损害作用,其机制可能是通过损害血脑屏障、促进神经元凋亡进而造成大鼠神经功能损伤。
Objective To investigate the primary mechanism of neurological dysfunction after blast injury in rats.Methods There were 32 healthy male SD rats were randomly divided into the control group and the blast injury group.The blast injury model was made with TNT explosion.The neurobehavioral score of the two groups were detected by Garcia scoring system.Immunofluorescence staining was used to determine the apoptosis of hippocampal neurons,and immunohistochemistry was used to examine the difference in the expression of ZO-1 protein in two groups.Results The neurobehavioral scores of rats in blast injury group were significantly lower than those in control group(P<0.05).The closer to the explosion center was,the lower the neurobehavioral score was.TUNEL test showed that the apoptosis rate of nerve cells in the blast injury group was significantly higher than that in the control group,and immunohistochemical results showed that the positive expression rate of ZO-1 protein in the blast injury group was significantly lower than that in the control group(P<0.05).Conclusion Blast injury has an obvious impairment effect on neurological function in rats,which may be caused by BBB damage and neuronal apoptosis.
作者
刘彤
任宪奔
李营
韩守孟
张为
李明昌
LIU Tong;REN Xianben;LI Ying(Department of Neurosurgery,Renmin Hospital of Wuhan University,Wuhan 430060,China)
出处
《临床外科杂志》
2020年第10期917-919,共3页
Journal of Clinical Surgery
基金
中央高校基本科研业务费专项资金项目(2042018gf0038)。
关键词
爆震伤
神经功能损伤
血脑屏障
blast injury
neurological dysfunction
blood-brain barrier
