摘要
选取清洁类、护理类、美容修饰类6种化妆品和7株代表菌株,开展了ATP生物荧光增幅法与《化妆品安全技术规范》(2015年版)中菌落总数、霉菌和酵母菌检验方法的部分等效性研究。将代表菌株按照目标浓度10,5,1和0.1 CFU/g分别接种于6种化妆品,每个浓度重复检测5次,经Fisher exact test统计分析发现ATP生物荧光增幅法的检测限显著优于标准方法(p=0.0001)。7株代表菌株在MTAT培养基中生长良好,ATP生物荧光增幅法检测人工污染样品结果均为阳性,表明该方法的专属性良好。选取3家不同的实验室,采用两种方法对相同产品进行比对检测,检测结果无统计学差异(p>0.05),表明该方法的重现性良好。选取不同品牌TAT肉汤配制的富集培养基MTAT进行增菌,ATP检测结果均为阳性,表明该方法的耐用性良好。因此,ATP生物荧光增幅法与标准方法具有部分等效性。
Six kinds of cosmetics,including cleanser,care cosmetics and makeup categories,and seven representative microorganisms were selected to conduct the partial equivalence study between amplified ATP bioluminescence assay and the detection method of aerobic bacterial count and molds and yeast count in Safety and Technical Standard for Cosmetics(2015th Edition).The seven representative microorganisms were inoculated into the six kinds of cosmetics at target concentrations of 10,5,1 and 0.1 CFU/g,respectively.Tests on each concentration were repeated five times.Fisher exact test statistical analysis show that the detection limit of amplified ATP bioluminescence assay is significantly better than that of the standard method(p=0.0001).Seven representative strains grew well in the MTAT medium,and the results of artificially contaminated samples detected by the amplified ATP bioluminescence assay were all positive,indicating that the method has good specificity.Three different laboratories were selected to compare the two methods using the same samples,and no statistical difference was found in the results,indicating the method is reproducible(p>0.05).The enrichment media MTAT,prepared with selected brands of TAT base medium,were assessed using growth promotion,and their ATP test results were all positive,indicating that the method is has good durability.Therefore,the amplified ATP bioluminescence assay is partially equivalent to the standard method.
作者
翟磊
葛媛媛
崔生辉
刘吉泉
洪海军
Annemieke Masolijn
姚粟
ZHAI Lei;GE Yuan-yuan;CUI Sheng-hui;LIU Ji-quan;HONG Hai-jun;Annemieke Masolijn;YAO Su(China National Research Institute of Food&Fermentation Industries Co.,Ltd.,Beijing 100015,China;National Institutes for Food and Drug Control,Beijing 100050,China;Procter&Gamble International Operations,Singapore 138547,Singapore;Unilever(China)Limited,Shanghai 200335,China;Charles River,Microbial Solutions,Maastricht 6199,Netherlands)
出处
《日用化学工业》
CAS
CSCD
北大核心
2020年第11期771-775,782,共6页
China Surfactant Detergent & Cosmetics
关键词
ATP生物荧光增幅法
化妆品
微生物检测
方法验证
amplified ATP bioluminescence assay
cosmetics
microbial detection
method validation
