摘要
从NCBI数据库中检索得到甘薯ANS序列,利用同源比对在前期转录组数据库中筛选获得同源序列,通过PCR反应克隆出甘薯ANS基因(IbANS)的c DNA全长序列,进而预测和分析IbANS基因的蛋白质序列,同时对其在不同甘薯品种的不同组织进行了实时定量PCR分析。结果表明,该c DNA具有最大开放阅读框(ORF),共1086个碱基,编码362个氨基酸残基;ANS蛋白为亲水性蛋白质,不存在信号肽;保守结构域预测分析表明,该基因编码的蛋白具有典型的ANS蛋白功能结构域,属于2OG-Fe II_Oxy双加氧酶超家族;系统进化树分析显示,甘薯ANS与三浅裂野牵牛亲缘关系最近;荧光定量PCR结果表明,IbANS在紫心甘薯块根中高表达。研究结果可为进一步阐明IbANS基因在紫心甘薯块根中的形成及累积机制提供理论基础。
The function of IbANS gene in anthocyanin biosynthesis pathway was studied to lay the foundation for molecular breeding of Ipomoea batatas.In this study,the full-length c DNA sequence of IbANS was cloned by PCR technology.Then the IbANS gene sequence and its functional domains were predicted and analyzed.Meanwhile,the expression level of IbANS gene was detected and analyzed in selected tissues.The results showed that the open reading frame(ORF)of this sequence was 1086,which encoded 362 amino acid residues.The ANS protein was a hydrophilic protein,and the protein had no signal peptide.Conservative domain indicated that the protein had typical functional domain of ANS protein and belonged to the superfamily of 2 OG-Fe II_Oxy dioxygenase.Phylogenetic tree showed that the ANS protein in Ipomoea batatas was similar to those in Ipomoea trifida.Real-time PCR results showed that IbANS was expressed in all selected tissues,with higher expression in tubers of purple-fleshed potato.The results of this study will provide a theoretical basis for further elucidating the formation and accumulation mechanism of IbANS gene in purple sweet potato tubers.
作者
杨慧珍
YANG Huizhen(College of Agronomy,Shanxi Agricultural University,Taiyuan 030031,China)
出处
《山西农业科学》
2020年第11期1718-1723,共6页
Journal of Shanxi Agricultural Sciences
关键词
ANS
甘薯
生物信息学分析
组织表达分析
ANS
Ipomoea batatas
bioinformatics analysis
tissue expression analysis
