摘要
目的探讨内质网氨肽酶(ERAP1)、肿瘤坏死因子受体Ⅰ(TNFR-Ⅰ)、肿瘤坏死因子受体Ⅱ(TNFR-Ⅱ)在寻常型银屑病皮损组织中的表达及意义。方法选取新疆维吾尔自治区人民医院皮肤科2015年至2017年期间经临床、病理明确诊断为寻常型银屑病的皮损石蜡组织标本共20例及15例正常皮肤组织,应用免疫组化Envision法检测其组织中ERAP1、TNFR-Ⅰ、TNFR-Ⅱ的表达,并检验其相关性。结果⑴正常皮肤组织中,ERAP1主要表达于表皮基底层;在寻常型银屑病皮损组织中,ERAP1呈弥漫阳性表达。寻常型银屑病皮损组织中ERAP1阳性表达的细胞数多于正常皮肤组织,两组间差异有统计学意义(Z=-4.170,P<0.001)。⑵TNFR-Ⅰ在正常皮肤组织呈弥漫阳性表达。在寻常型银屑病皮损组织表皮中,TNFR-Ⅰ主要表达于表皮棘层,表现为上强下弱型,棘层的表达较基底层强,两组间表达差异有统计学意义(χ2=17.740,P<0.001)。⑶正常皮肤组织中,TNFR-Ⅱ不表达;在寻常型银屑病皮损组织表皮中,TNFR-Ⅱ在基底细胞层及棘层呈不同程度弥漫阳性表达,两组之间的表达差异有统计学意义(χ2=17.500,P<0.001)。⑷寻常性银屑病皮损组织表皮中ERAP1的表达与TNFR-Ⅰ呈负相关(rs=-0.662,P=0.001);ERAP1的表达与TNFR-Ⅱ无明显相关性(P=0.139)。结论 ERAP1、TNFR-Ⅰ、TNFR-Ⅱ可能在寻常型银屑病的发病机制中起重要作用,它们之间可能相互作用,调节靶细胞增殖和凋亡,以维持寻常型银屑病表皮的异常增殖及良性增生状态。
Objective To explore the possible role of endoplasmic reticulum aminopeptidase I(ERAP1),tumor necrosis factor-a receptor Ⅰ(TNFR-Ⅰ),tumor necrosis factor-a receptor Ⅱ(TNFRⅡ)in the pathogenesis of psoriasis vulgaris.Methods 15 cases of normal skin and 20 cases of psoriasis vulgaris in Xinjiang Uygur Autonomous Region People's hospital were enrolled.Immunohistochemical staining techniques were used to detect the expression of ERAP1,TNFR-Ⅰ,TNFR-Ⅱ protein in the two groups.Statistical analysis was then performed to compare the difference in expression between the two groups.Results(1)In normal skin,ERAP1 is mainly expressed in the basal layer of the epidermis.In the psoriatic vulgaris lesions,ERAP1 was diffusely positive.The expression of ERAP1 in the psoriatic lesions was stronger than that of normal tissues,with stastically significant difference(Z=-4.170,P<0.05).(2)TNFR-Ⅰ is diffusedly expressed in the normal skin tissues.However,in the epidermis of psoriatic lesions,the expression of TNFR-Ⅰ in the spinous layer was stronger than that of the basal layer(χ^2=17.740,P<0.05).(3)In the normal skin,TNFR-Ⅱ was not expressed;in the psoriatic lesions,TNFRⅡ was diffusely expressed in the basal cell layer and acanthosis of the epidermis in varying degrees,and the difference between normal and psoriatic skin was statistically significant(χ^2=17.5(X),P<0.001).(4)The expression of ERAP1 in the epidermis of psoriasis vulgaris was negatively correlated with TNFR-Ⅰ(rs=-0.662,P=0.001).There was no significant correlation between ERAP1 and TNFR-Ⅱ(rs=0.343,P=0.139).Conclusions ERAP1,TNFR-Ⅰ,and TNFR-Ⅱ may play important roles in the pathogenesis of psoriasis vulgaris.They may interact with each other to regulate the proliferation and apoptosis of keratinocyte in order to maintain the abnormal proliferation and benign proliferation of psoriasis vulgaris epidermis.
作者
陈建霞
刘新梅
刘建勇
王鹏
陈小敏
马庆宇
冯燕艳
Chen Jianxia;Liu Xinmei;Liu Jianyong;Wang Peng;Chen Xiaomin;Ma Qingyu;Feng Yanyan(Department of Dermatology,Peoples Hospital of Xinjiang Uygur Autonomous Region,Urumqi,830001,China;Department of Dermatology,the Second Peoples Hospital of Chengdu,Chengdu 610000,China)
出处
《中国医师杂志》
CAS
2020年第10期1488-1492,共5页
Journal of Chinese Physician
基金
新疆维吾尔自治区自然科学基金(2016D01C102)
