摘要
外泌体是一种由细胞分泌的细胞外囊泡,其广泛存在于各类生物液中,是细胞间信息交流的途径之一。由于外泌体内携带的核酸、蛋白质及脂质等功能性物质可作为生物标记用于动物生理诊断,也可作为信息传输载体用于调节动物生理状态以及治疗动物疾病,因而受到广泛的研究,开发潜力巨大。建立成熟稳定且方便快捷的外泌体分离手段是保证外泌体相关研究顺利开展的前提。以下对当前动物外泌体分离的主要方法进行了综述,比较了不同分离手段的特点,以此为相关技术的进一步开发与后续的动物外泌体研究提供方法及理论参考。
Exosomes,widely existing in various biological fluids,are one type of extracellular vesicles secreted by cells and one of carriers of cell-cell communication.The functional materials carried by exosomes,such as nucleic acids,proteins and lipids,are considered as biomarkers for animal physiological diagnosis and as communication carriers for animal physiological states regulation or diseases treatment.Exosomes have been extensively studied as their great development potential.The establishment of mature,stable,convenient and fast exosome isolation methods is a prerequisite for ensuring the smooth progress of exosome related research.Summarized current main methods of animal exosome isolation and compared the characteristics of different isolation methods,the following provides methodological and theoretical references for the further development of related technologies and subsequent animal exosome research.
作者
邱金戈
刘德武
孙宝丽
李耀坤
郭勇庆
邓铭
柳广斌
QIU Jin-ge;LIU De-wu;SUN Bao-li;LI Yao-kun;GUO Yong-qing;DENG Ming;LIU Guang-bin(College of Animal Science,South China Agricultural University,Guangzhou 510642,China)
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2020年第9期36-42,共7页
China Biotechnology
基金
广东省畜禽地方品种保护与开发利用提升工程(2018-143)
广东省现代农业产业技术体系(2019KJ127)
阳江市科技计划(2018032)资助项目。
