摘要
为了研究巴戟天提取物不同极性萃取相的抗氧化及抑制α-葡萄糖苷酶活性,确定巴戟天主要活性部位,促进药食两用植物巴戟天在天然氧化剂和降血糖食物方面的应用。测量了巴戟天提取物不同极性萃取相的总多酚、总黄酮含量,并采用1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力、磷钼酸还原能力、羟自由基(·OH)清除能力作为指标测定其各部位体外抗氧化能力;并以阿卡波糖为阳性对照测定各部位抑制α-葡萄糖苷酶能力。结果表明,巴戟天提取物不同极性萃取相均有一定的抗氧化、降血糖活性,并在一定浓度范围内呈线性相关;氯仿部位总多酚和总黄酮含量最高,分别为(514.97±2.9)和(46.3±1.21) mg/g,其体外抗氧化及抑制α-葡萄糖苷酶活性也最强,且降血糖活性强于阳性对照阿卡波糖,在高浓度抑制率达97.9%。实验结果表明,巴戟天提取物氯仿部位具有良好的体外抗氧化、降血糖活性,为开发巴戟天作为天然抗氧化剂和降血糖食品提供了依据。
The paper aimed to study the antioxidation and inhibition ofα-glucosidase activity of M.officinalis How extract in different polar extraction phase,determine the main active site of M.officinalis How,and promote the application of M.officinalis How in natural oxidant and hypoglycemic food.The contents of total polyphenols and flavonoids in different polar extraction phases of M.officinalis How extract were measured,and the antioxidant capacity in vitro was measured by DPPH free radical scavenging capacity,phosphomolybdic acid reducing capacity and hydroxyl free radical scavenging capacity,and the inhibitory capacity ofα-glucosidase was measured by acarbose as positive control.The results showed that the extracts of M.officinalis How had some antioxidant and hypoglycemic activities in different polar extraction phases,and showed linear correlation within a certain concentration range;the highest content of total polyphenols and flavonoids in chloroform were(514.97±2.9)and(46.3±1.21)mg/g,respectively,and their anti-oxidation and inhibition ofα-glucosidase activities in vitro were also the strongest,and hypoglycemic activities were stronger than those in positive control ACA.The inhibition rate of bosaccharide in high concentration was 97.9%.The results showed that the chloroform part of M.officinalis How extract had good antioxidant and hypoglycemic activities in vitro,which provided a basis for the development of M.officinalis How as a natural antioxidant and hypoglycemic food.
作者
曾铁鑫
姚志仁
李豫
朱开梅
兰圆圆
顾生玖
ZENG Tiexin;YAO Zhiren;LI Yu;ZHU Kaimei;LAN Yuanyuan;GU Shengjiu(School of Pharmacy,Guilin Medical College,Guilin 541100,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第19期192-196,共5页
Food and Fermentation Industries
基金
广西创新驱动发展专项基金项目(桂科AA18118015)
广西科学研究与技术开发计划项目(桂科合14123001-22,桂科能1598025-46)
广西省桂林市科学研究与技术开发计划项目(20150102-6,20150102-7,20150102-8)
广西植物功能物质研究与利用重点实验室开放基金项目(FPRU2015-5)
广西科学院基本科研业务费资助项目(2017YJJ23010)。
关键词
巴戟天
总多酚
总黄酮
抗氧化活性
降血糖活性
Morinda officinalis How
total polyphenols
total flavonoids
antioxidant activity
hypoglycemic activity
