摘要
目的:检测miR-34c-3p与增殖细胞核抗原(PCNA)在三阴性乳腺癌(TNBC)组织和血清中的表达,分析二者的相关性,探讨其在TNBC发病中的意义。方法:选择2017年12月至2019年12月于空军军医大学第一附属医院就诊的72例TNBC患者作为实验组(A组),以同期就诊的60例乳腺纤维腺瘤或乳腺腺病患者为对照组(B组)。采用Real-time RT-PCR检测乳腺组织标本中miR-34c-3p m RNA及PCNA m RNA的表达;免疫组化检测两组乳腺组织中PCNA蛋白表达;ELISA法检测血清中PCNA的表达。并进一步分析癌组织中miR-34c-3p m RNA的表达与癌组织中PCNA m RNA及血清PCNA水平的相关性。结果:A、B组miR-34c-3p m RNA的相对表达量分别为0.884±0.159及1.255±0.131,A组明显低于B组,差异有统计学意义(P<0.001)。A、B组PCNA m RNA的相对表达量分别为1.544±0.215及1.230±0.204,A组表达高于B组,差异有统计学意义(P<0.001)。免疫组化检测PCNA蛋白表达,A组阳性例数为50例,阴性例数为22例;B组阳性例数为21例,阴性例数为39例;A组阳性率明显高于B组,差异有统计学意义(P<0.001)。两组患者血清中PCNA的表达,A组[(383.194±110.832)pg/ml]明显高于B组[(257.867±101.697)pg/ml],差异有统计学意义(P<0.001)。将A组TNBC患者肿瘤组织miR-34c-3p m RNA表达结果分别与PCNA m RNA表达结果及其血清中PCNA表达结果进行Pearson相关分析,结果显示:miR-34c-3p m RNA与PCNA m RNA及血清PCNA均呈明显负相关关系(P<0.001、P=0.024<0.05)。结论:TNBC组织中miR-34c-3p表达下调,PCNA在癌组织及血清中均表达上调,二者在TNBC患者组织和血清中呈明显负相关关系。miR-34c-3p与PCNA可能在TNBC的发病中起着重要的作用。
Objective:To detect the expression of miR-34 c-3 p and PCNA in triple negative breast cancer(TNBC)tissues and serum,analyze the relationship between miR-34 c-3 p and PCNA,explore the significance of miR-34 c-3 p and PCNA in TNBC.Methods:72 TNBC patients in the First Affiliated Hospital of Air Force Medical University from December 2017 to December 2019 were selected as the experimental group(group A).60 patients with fibroadenoma or adenosis of breast in the same period were taken as the control group.The expression of miR-34 c-3 p mRNA and PCNA mRNA in breast tissue by Real time RT-PCR.Expression of PCNA protein in breast tissues of two groups was detected by immunohistochemical detection.The expression of PCNA in serum was detected by ELISA.Then,the correlation between the expression of miR-34 c-3 p mRNA and the levels of PCNA mRNA and serum PCNA in cancer tissues was analysed.Results:The relative expression of miR-34 c-3 p mRNA in group A and B was 0.884±0.159 and 1.255±0.131,respectively.Group A was significantly lower than group B,and the difference was statistically significant(P<0.001).The relative expression of PCNA mRNA in group A and B was 1.544±0.215 and 1.230±0.204,respectively.Group A was significantly higher than group B,and the difference was statistically significant(P<0.001).The expression of PCNA protein was detected by immunohistochemistry.The number of positive cases in group A was 50,and the number of negative cases was 22.The number of positive cases in group B was 21,and the number of negative cases was 39.The positive rate in group A was significantly higher than that in group B,and the difference was statistically significant(P<0.001).The expression of PCNA in serum in group A[(383.194±110.832)pg/ml]was significantly higher than in group B[(257.867±101.697)pg/ml],and the difference was statistically significant(P<0.001).The expression of miR-34 c-3 p mRNA and PCNA mRNA in TNBC tumor tissue and serum PCNA of group A were analyzed by Pearson correlation analysis.The results showed that miR-34 c-3 p mRNA was negatively correlated with PCNA mRNA and serum PCNA,respectively(P<0.001,P=0.024<0.05).Conclusion:The expression of miR-34 c-3 p was down-regulated in TNBC,and PCNA was up-regulated in cancer tissue and serum.miR-34 c-3 p and PCNA may play an important role in the pathogenesis of TNBC.
作者
巫姜
石文龙
牛进
杨玉庆
孟庆杰
赵戈
李南林
凌瑞
Wu Jiang;Shi Wenlong;Niu Jin;Yang Yuqing;Meng Qingjie;Zhao Ge;Li Nanlin;Ling Rui(Department of Thyroid Breast and Vascular Surgery,the Affiliated Hospital of Air Force Medical University,Shaanxi Xi'an 710032,China;Department of General Surgery,CITIC Machinery Manufacturing Inc General Hospital,Shanxi Wenxi 043801,China;Department of Thyroid and Breast Surgery,Xi'an No.3 Hospital,Affiliated Hospital of Northwest University,Shaanxi Xi'an 710082,China)
出处
《现代肿瘤医学》
CAS
2020年第20期3534-3540,共7页
Journal of Modern Oncology
基金
国家自然科学基金(编号:81472598)。
