摘要
利用抗病基因改良品种的抗病性是防治条锈病危害的有效途径,育种过程中使用分子标记辅助选择(MAS,markerassisted selection)能够有效提高目标基因的选择效率。为了检测长4738/CH7086和烟农999/CH7086后代选系中是否含有抗条锈病基因Yr69并验证Yr69连锁标记的有效性,利用分子标记2AS33和人工接种鉴定的方法对2个群体的63个F6品系进行了标记检测和抗性评价。结果表明,长4738/CH7086后代的35个品系中有32个含有Yr69,且全部表现为抗病,1个品系未检测到Yr69但也表现为抗病;烟农999/CH7086后代的28个品系中有8个含有Yr69,其中7个抗性稳定,1个出现抗、感分离现象;63个品系中有3个基因型与表型不一致,2AS33检测的准确率为95.2%,可有效用于抗条锈病基因Yr69的分子标记辅助选择。筛选出的40个稳定抗病品系经过产量与适应性鉴定后可用于参加品种比较试验,也可作为优良亲本用于抗病育种。
One of the effective ways to control the damage of stripe rust in wheat is the introduction of stripe rust resistant genes into cultivars to improve their resistance.Molecular marker-assisted selection(MAS)can improve the selection efficiency of target genes in breeding.In order to detect the stripe rust resistant gene Yr69 and verify the validity of molecular marker linked to Yr69,the genotypes of 63 F6 lines derived from Chang 4738/CH7086 and Yannong 999/CH7086 were identified by molecular marker 2AS33,and their resistance to stripe rust were identified with the Pst mixed with CYR32,CYR32 and CYR34.The results showed that 32 lines among the 35 lines derived from Chang 4738/CH7086 had Yr69 and showed resistance to stripe rust,and one line showed resistance to stripe rust without Yr69 being detected;8 lines among the 28 lines derived from Yannong 999/CH7086 had Yr69,of which 7 lines showed homozygous resistance and one line showed segregation of resistance and susceptibility.Among the total 63 lines,3 had genotypes inconsistent with phenotypes.It suggested that the marker 2AS33 had a high accuracy of 95.2%and it could be used to detect the Yr69 gene effectively in molecular marker-assisted selection breeding.The 40 resistant lines identified,after yield and adaptability identification,may be entered for regional trials of cultivars directly or as good parents for stripe rust resistance breeding.
作者
张晓军
杨文静
郭慧娟
闫贵云
李欣
乔麟轶
常利芳
阎晓涛
张树伟
郭数进
贾举庆
畅志坚
ZHANG Xiao-jun;YANG Wen-jing;GUO Hui-juan;YAN Gui-yun;LI Xin;QIAO Lin-yi;CHANG Li-fang;YAN Xiao-tao;ZHANG Shu-wei;GUO Shu-jin;JIA Ju-qing;CHANG Zhi-jian(College of Agronomy,Shanxi Agricultural University/Shanxi Key Laboratory of Crop Genetics and Molecular Improvement,Taigu 030801)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2020年第5期1295-1300,共6页
Journal of Plant Genetic Resources
基金
国家重点研发计划(2017YFD0100600)
山西省重点研发计划(201903D211003,201803D221018-5,201803D421020,201903D211073,201803D221008-2)
山西省重点科技创新平台(201605D151002)
关键词
小麦
抗条锈病基因
Yr69
分子标记
育种
wheat
stripe rust resistance gene
Yr69
molecular marker
breeding
