摘要
【目的】明确棉蚜Aphis gossypii Glover体内3个高表达谷胱甘肽-S-转移酶(GST)基因不同发育阶段的表达谱,并比较它们在不同寄主专化型棉蚜中的表达差异。【方法】从棉蚜的伏蚜和苗蚜转录组数据库中挑选GSTs并克隆得到全长cDNA序列,使用qRT-PCR分析这些基因在不同寄主专化型棉蚜不同龄期中的相对表达量。【结果】克隆到3个高表达的GSTs基因,分别命名为AgoGST-s1(GenBank登录号:MN688789)、AgoGST-d1(GenBank登录号:MN688790)、AgoGST-d2(GenBank登录号:MN688791)。这3个基因在不同龄期均表达,不同发育阶段的表达谱基本一致,表达量呈现从低到高的变化趋势。AgoGST-s1基因在各个发育阶段相对表达量的变化幅度较小,而AgoGST-d1、AgoGST-d2的变化幅度较大。对比两种寄主专化型棉蚜相同龄期时的表达情况发现,黄瓜型棉蚜中GSTs基因的表达量在大部分龄期都高于棉花型棉蚜。【结论】AgoGST-s1、AgoGST-d1、AgoGST-d2基因表达量伴随棉蚜生长发育变化趋势明显,在不同寄主专化型棉蚜体内的表达量存在差异,可能与棉蚜对寄主植物的适应性有关。
[Objectives] To determine the profiles of three high expression glutathione-S-transferase(GST) genes in various developmental stages of Aphis. gossypii Glover, and compare the expression of these genes in different host-races of this pest. [Methods] Full-length cDNA sequences of GSTs genes were cloned based on transcriptome data of A. gossypii specimens collected from seedling and mid-summer growth stages of cotton. The relative expression levels in different developmental stages(nymph and adult) and host-races(cucumber and cotton host-races) were investigated with real-time quantitative PCR. [Results] Three high expression GSTs were identified and named AgoGST-s1(GenBank accession no.: MN688789), AgoGST-d1(GenBank accession no.: MN688790) and AgoGST-d2(GenBank accession no.: MN688791). These 3 genes were expressed in all development stages and their temporal expression profiles all tended to increase over time. The relative expression of AgoGST-s1 gene was less in all developmental stages, whereas that of AgoGST-d1 and AgoGST-d2 was more variable. Expression of these 3 GSTs in the cucumber host-race was higher than in the cotton host-race for most age-classes. [Conclusion] Expression of the AgoGST-s1, AgoGST-d1 and AgoGST-d2 genes is significantly associated with the developmental stage of A. gossypii. The expression patterns of these 3 GST genes in two host-races differed, which may reflect adaptation to different host plants.
作者
王传鹏
张帅
高雪珂
雒珺瑜
朱香镇
王丽
张开心
杨亦桦
崔金杰
WANG Chuan-Peng;ZHANG Shuai;GAO Xue-Ke;LUO Jun-Yu;ZHU Xiang-Zhen;WANG Li;ZHANG Kai-Xin;YANG Yi-Hua;CUI Jin-Jie(College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China;State Key Laboratory of Cotton Biology,Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang 455000,China)
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2020年第4期823-832,共10页
Chinese Journal of Applied Entomology
基金
国家棉花产业技术体系(CARS-15-20)。
关键词
棉蚜
谷胱甘肽-S-转移酶
发育阶段
寄主专化型
表达谱
Aphis gossypii
glutathione-S-transferase(GST)
host specialization
development stage
expression profile
