摘要
目的研究不同剂量60Co-γ射线辐照灭菌对水蛭药粉成分及卫生学指标的影响,为水蛭灭菌工艺提升提供依据。方法使用不同剂量60Co-γ射线对水蛭药粉进行辐照灭菌,对灭菌前后的水蛭的水分、总灰分、酸不溶性灰分、pH值、重金属及有害元素、黄曲霉毒素、抗凝活性等药典要求检查项进行测定,以及对微生物含量等卫生学指标进行测定,并对不同剂量60Co-γ射线辐照灭菌的水蛭药粉进行化学成分HPLC图谱分析。结果不同剂量60Co-γ射线辐照前后水蛭药粉中的水分、总灰分、酸不溶性灰分、pH值、重金属及有害元素、黄曲霉毒素和抗凝活性无明显差异;水蛭药粉在不同剂量60Co-γ射线辐照灭菌前后,各试验组之间卫生学指标差异明显,8 kGy及以上的60Co-γ射线动态辐照可对微生物进行有效杀灭;高效液相色谱测定灭菌前后7种化学成分无明显差异,灭菌前后质量稳定。结论60Co-γ射线辐照可有效杀灭水蛭药粉中微生物,8~10 kGy的剂量效果最佳,辐照灭菌前后药粉药典检查项无明显差异,辐照后的药粉品质更优。
Objective To study the effect of 60Co-γ ray irradiation sterilization on the composition and hygiene index of leech medicine powder,and provide a basis for the improvement of leech sterilization process.Methods Irradiation sterilization of leech powder with different doses of 60Co-γ rays,measure moisture,total ash,acid-insoluble ash,pH value,heavy metals and harmful elements,aflatoxin,anticoagulant activity according to pharmacopoeia requirements and other hygienic indicators such as microbial content before and after sterilization.The chemical constituents of leech powder sterilized by irradiation with different doses of 60Co-γ rays were analyzed by HPLC.Results There were no significant difference in moisture,total ash,acid-insoluble ash,pH value,heavy metals and harmful elements,aflatoxin and anticoagulant activity in the peony powder before and after irradiation with different doses of 60Co-γ rays.Before and after sterilization with different doses of 60Co-γ ray irradiation,the hygienic parameters of the experimental groups were significantly different.Dynamic irradiation with 60Co-γ rays at 8 kGy and above could effectively kill microorganisms.There were no significant differences in 7 chemical components before and after sterilization by HPLC,and the quality was stable before and after sterilization.Conclusions 60Co-γ ray irradiation can effectively kill microorganisms in medicinal powder,and the dose effect of 8~10 kGy is the best.There is no significant difference in the pharmacopoeia check items before and after irradiation sterilization,and the quality of the powder after irradiation is better.
作者
张微
韩振明
杨梅
关永霞
马云
杜昊忱
张贵民
ZHANG Wei;HAN Zhenming;YANG Mei;GUAN Yongxia;MA Yun;DU Haochen;ZHANG Guimin(Lunan Houpu Pharmaceutical Co.,Ltd.,Linyi 276006 China;State Key Laboratory of Generic Manufacture Technology of Traditional Chinese Medicine;Lunan Pharmaceutical Group Co.,Ltd.)
出处
《中国辐射卫生》
2020年第3期207-210,217,共5页
Chinese Journal of Radiological Health
基金
临沂市兰山区科技创新发展计划专项(1811)
临沂市自主创新重大专项(2019ZDZX001)。
关键词
60Co-γ射线
辐照灭菌
水蛭
品质
60Co-γRays
Irradiation Sterilization
Whitmania Pigra
Quality
