摘要
目的基于网络药理学探讨黄芪治疗冠心病的机制。方法应用TCMSP、DRAR-CPI、UniprotKB、GeneCards数据库预测黄芪主要活性成分及其治疗冠心病的作用靶点。应用Cytoscape软件,构建黄芪—冠心病疾病靶点群网络;并对靶点GO富集分析和KEGG信号通路进行分析。结果黄芪对冠心病发挥治疗作用涉及19个活性成分和107个潜在作用靶点,其中主要包括调节凝血过程靶点(PROC、VDR、F9、ITGAL)、脂代谢调控靶点(RXRA、PLA2G2A、PPARD、PPARA、NR1H4)以及氧化应激调控靶点(GSTT1、SOD1)等。GO分析发现黄芪参与调控甾体激素、细胞对脂多糖反应、血管舒张的正向调节等生物学过程;KEGG分析结果显示黄芪参与调控补体和凝血级联反应、胰岛素抵抗、缺氧诱导因子1信号通路、细胞色素P450药物代谢等信号途径。结论黄芪治疗冠心病的作用机制主要包括凝血、脂代谢及抗氧化损伤过程调节。本研究为为黄芪治疗冠心病的基础与临床研究提供一定参考。
Objective To explore the mechanism of astragalus membranaceus in the treatment of coronary heart disease(CHD)based on network pharmacology.Method By means of TCMASP,DRAR-CPI,UniproKB,and GeneCards database,the main active components of astragalus membranaceus and the target of the treatment of coronary heart disease were predicted.The target group network of astragalus membranaceus and coronary heart disease was constructed by Cytoscape software.Finally,the target GO enrichment analysis and the KEGG signal path were analyzed.Result There were 19 active components and 107 potential targets in astragalus membranaceus for CHD,which mainly involved regulating coagulation process(PROC、VDR、F9、ITGAL),lipid metabolism(RXRA、PLA2 G2 A、PPARD、PPARA、NR1 H4),and anti-oxidative damage(GSTT1、SOD1).GO analysis showed that astragalus membranaceus was involved in the regulation of steroid hormone mediated,cell response to lipopolysaccharide,positive regulation of vasodilation and so on.KEGG analysis showed that astragalus membranaceus was involved in the regulation of complement and coagulation cascade reaction,insulin resistance,hypoxia-inducing factor 1 signaling pathway,cytochrome P450 drug metabolic pathway and so on.Conclusion The mechanism of astragalus membranaceus in treating CHD mainly includes coagulation,lipid regulation and anti-oxygen injury process.This study provides references for the treatment of coronary heart disease with astragalus membranaceus.
作者
彭瑜
王永祥
秦红岩
臧凯宏
PENG Yu;WANG Yong-xiang;QIN Hong-yan;ZANG Kai-hong(Department of Heart Center,The First Hospital of Lanzhou University,Lanzhou,730000;Key Laboratory of Cardiovascular Diseases of Gansu Province,Lanzhou,730000;Department of Pharmacy,The First Hospital of Lanzhou University,Lanzhou,730000;College of Pharmacy,Gansu University of Chinese Medicine,Lanzhou,730000)
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2020年第8期935-940,共6页
Chinese Journal of Integrated Traditional and Western Medicine
基金
科技部国家重点研发计划项目(No.2018YFC1311505)
甘肃省心血管疾病重点实验室建设项目(No.1206RTSA025)
2020兰州大学中央高校基本科研专项资金(No.lzujbky-2020-kb18)
甘肃省心血管病临床医学研究中心(No.甘科技[2018]20号)
重大疑难病中西医临床协作试点项目(No.国中医药办政发[2018]3号)。
关键词
黄芪
冠心病
网络药理学
凝血过程
脂代谢
氧化损伤
astragalus membranaceus
coronary heart disease
network pharmacology
coagulation process
lipid metabolism
anti-oxidative damage
