摘要
目的基于16S rRNA基因测序,探讨鱼藤酮诱导的帕金森病(PD)模型小鼠体内肠道微生物的变化。方法14只8周雄性C57BL/6J小鼠连续5周每天皮下注射鱼藤酮(3 mg/kg),每周测1次体重,5周后进行行为学检测,包括转棒实验和旷场实验,最后收集肠道内容物,用于肠道微生物检测分析。结果给鱼藤酮5周后,PD模型小鼠的体重显著低于对照小鼠(P<0.05),PD模型小鼠在转棒中的运动时间(P<0.0001)和在旷场中的运动总路程(P<0.05)均显著低于对照小鼠。PD模型小鼠肠道微生物Alpha多样性没有变化(P>0.05),但是微生物物种与对照组间差异有显著性,其中PD模型小鼠肠道铜绿假单胞菌(Turicibacter)显著减少(P<0.01),毛螺旋菌科(norank_f_Lachnospiraceae)显著增多(P<0.01),丹毒丝菌科(norank_f_Erysipelotrichaceae)显著减少(P<0.01),腔隙杆菌属(Lachnoclostridium)显著增多(P<0.01)。结论PD模型小鼠的肠道微生物紊乱;肠道菌群紊乱可能参与PD模型小鼠运动障碍的发生。
Objective To investigate the changes of intestinal microbes in rotenone-induced Parkinson’s disease(PD)mice based on 16 S rRNA gene sequencing.Methods Fourteen 8-week-old male C57 BL/6 J mice were randomly divided into two groups:6 mice in the control group and 8 mice in the model group.The model mice were injected subcutaneously with rotenone(3 mg/kg)for 5 weeks,and the body weight was measured once a week.After 5 weeks,behavioral tests were performed,including the rotating rod test and the open field test.The contents of the tract were used for intestinal microbial detection analysis.Results After 5 weeks of rotenone treatment,the weight of PD mice was significantly lower than that of the control mice(P<0.05).The movement time of the PD model mice in the rotating rod(P<0.0001)and the total distance of movement in the open field(P<0.05)were significantly lower than that of the control mice.In addition,the intestinal microbial diversity of the PD model mice did not change(P>0.05),but the microbial species showed significant differences.Among them,the PD mice showed a significant decrease in the intestinal Turicibacter(P<0.01),a significant increase in norank_f_Lachnospiraceae(P<0.01),a significant decrease in norank_f_Erysipelotrichaceae(P<0.01),and a significant increase in Lachnoclostridium(P<0.01).Conclusion Intestinal microbes in PD mice are disordered,and these intestinal flora may be involved in the development of dyskinesia in PD mice.
作者
韩秋琴
万国庆
顾雪锋
HAN Qiu-qin;WAN Guo-qing;GU Xue-feng(Research Department,Affiliated Zhoupu Hospital,Shanghai University of Medicine and Health Sciences,Shanghai 201318,China)
出处
《解剖学报》
CAS
CSCD
北大核心
2020年第4期507-512,共6页
Acta Anatomica Sinica
基金
上海市教育委员会高校青年教师培养资助计划(ZZJKYX19006,ZZJKYX19007,ZZJKYX19009)。
