摘要
目的:探讨肿瘤坏死因子相关凋亡诱导体(TRAIL)与孕酮受体膜组件1(PGRMC1)在胎膜早破(PROM)孕妇胎膜组织中的表达水平及临床意义。方法:抽取产科因足月PROM行剖宫产术结束妊娠的60例孕妇(PROM组),分娩后胎盘病理检查是否伴有绒毛膜羊膜炎(CAM);另抽取同期行剖宫产分娩的60例健康孕妇(对照组),均以免疫组织化学法、Western blotting法检测胎膜组织中TRAIL、PGRMC1蛋白表达情况。结果:TRAIL、PGRMC1均主要表达于胎膜的绒毛膜层与蜕膜层。与对照组相比,PROM组孕妇胎膜组织中TRAIL阳性表达率明显升高(P<0.01),PGRMC1表达率明显降低(P<0.01);且胎膜组织中TRAIL蛋白相对表达量明显升高(P<0.01),PGRMC1蛋白相对表达量明显降低(P<0.01)。PROM孕妇中发生CAM者胎膜组织TRAIL、PGRMC1阳性表达率与未发生CAM者相比差异无统计学意义(P>0.05),而发生CAM者TRAIL蛋白相对表达量明显高于未发生CAM者(P<0.01),PGRMC1蛋白相对表达量明显低于未发生CAM者(P<0.01)。结论:PROM孕妇胎膜组织中存在TRAIL蛋白高表达,PGRMC1蛋白低表达,二者在PROM发病中发挥重要作用,且与CAM有一定关联,可作为临床检查诊断的参考指标。
Objective:To investigate the expression levels and clinical significance of tumor necrosis factor-related apoptosis inducing ligand(TRAIL)and progesterone receptor membrane component 1(PGRMC1)in fetal membrane tissues of pregnant woman with premature rupture of membranes(PROM).Methods:Sixty pregnant women treated with cesarean section because of full-term PROM to stop the pregnancy in obstetrics department were set as the PROM group.After delivery,the chorioamnionitis(CAM)in PROM group were identified using placenta pathological examination.Sixty healthy pregnant women treated with cesarean delivery in the same period were set as the control group.The expression levels of TRAIL and PGRMC1 protein in fetal membrane tissues were detected using the immunohistochemistry and Western blotting,and analyzed using statistical method.Results:The TRAIL and PGRMC1 were mainly expressed in chorion and decidua layers of membranes.Compared with the control group,the positive expression rates of TRAIL and PGRMC1 in fetal membrane tissues in PROM group significantly increased and decreased,respectively(P<0.01).The relative expression levels of TRAIL and PGRMC1 protein in fetal membrane tissues in PROM group significantly increased and decreased compared with the control group,respectively(P<0.01).The differences of the positive expression rates of the TRAIL、PGRMC1 in fetal membrane tissues of PROM pregnant women with or without CAM were not statistically significnat(P>0.05).The relative expression level of TRAIL protein in patients with CAM was significantly higher than that in patients without CAM(P<0.01),while the relative expression level of PGRMC1 in patients with CAM was significantly lower than that in patients without CAM(P<0.01).Conclusions:The expression levels of TRAIL and PGRMC1 proteins in fetal membrane tissues of PROM pregnant women are high and low,both play an important role in the pathogenesis of PROM,and are related to CAM,which can be used as a reference index for clinical examination and diagnosis.
作者
张静
张成
唐国珍
马俊如
裴娇娇
吴君
罗希
谭春梅
ZHANG Jing;ZHANG Cheng;TANG Guo-zhen;MA Jun-ru;PEI Jiao-jiao;WU Jun;LUO Xi;TAN Chun-mei(Department of Obstetrics,The First Affiliated Hospital of Chengdu Medical College,Chengdu Sichuan 610500,China)
出处
《蚌埠医学院学报》
CAS
2020年第7期876-879,共4页
Journal of Bengbu Medical College
基金
四川省干部保健科研课题(川干研2016-706)。
关键词
胎膜早破
肿瘤坏死因子相关凋亡诱导体
孕酮受体膜组件1
胎膜组织
premature rupture of membrane
tumor necrosis factor-related apoptosis inducing ligand
progesterone receptor membrane component 1
fetal membrane tissue
