摘要
目的探讨TRPC3基因多态性与原发性高血压患者左心室肥厚(LVH)的关系。方法305例原发性高血压患者根据左心室质量指数(LVMI)分为单纯高血压224例(对照组)和高血压合并LVH 81例(LVH组)。分析2组年龄、吸烟史等一般资料差异。留取外周血标本,采用Sequenom MassARRAY®SNP检测技术对TRPC3基因单核苷酸多态性(SNP)位点Rs2292232、Rs4995894、Rs4292355进行基因分型。Logistic回归分析影响患者LVH的因素。结果(1)2组年龄、吸烟史、饮酒史、家族史、尿素、肌酐、空腹血糖、三酰甘油、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、24 h平均舒张压(24 h DBP)差异无统计学意义(P>0.05),LVH组较对照组男性比例降低,而24 h平均收缩压(24 h SBP)、体质量指数(BMI)升高(P<0.05)。(2)2组Rs4995894及Rs4292355位点等位基因频率、基因型频率分布差异无统计学意义(P>0.05),TRPC3基因SNP位点Rs2292232基因型频率和等位基因频率分布差异有统计学意义(P<0.05),其中LVH组Rs2292232位点TT基因型频率较对照组更高(P<0.05)。(3)Logistic回归分析显示,女性(OR=0.332,95%CI:0.181~0.610)、较高24 h SBP(OR=1.035,95%CI:1.014~1.056)、TRPC3基因SNP Rs2292232 TT基因型(OR=2.105,95%CI:1.109~3.995)为原发性高血压患者发生LVH的独立危险因素(均P<0.05)。结论TRPC3基因Rs2292232位点SNP与高血压患者发生LVH有关,其中TT基因型患者更易发生LVH。
Objective To study the relationship between gene polymorphism of TRPC3 and left ventricular hypertrophy(LVH)inpatients with essential hypertensive(EH).Methods Genomic DNA was isolated from blood of 305 EH patients,including 205 male patients and 100 female patients.According to the left ventricular mass index(LVMI),the patients were divided into simple hypertension group(normal group,n=224)and EH+LVH group(n=81).The differences in general data such as age and smoking history were analyzed between the two groups.The single nucleotide polymorphism(SNP)sites Rs2292232,Rs4995894 and Rs4292355 of TRPC3 gene were genotyped by Sequenom MassARRAY®SNP detection technology.Logistic regression analysis was used for analyzing factors affecting LVH inpatients.Results(1)There were no significant differences in age,smoking history,drinking history,family history,urea,creatinine,fasting blood glucose,triglycerides,total cholesterol,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol and 24-hour mean diastolic blood pressure(24 h DBP)between the two groups.The male proportion was lower in EH+LVH group than those of normal group.Values of 24 h DBP and body massindex(BMI)were higher in EH+LVH group than those of normal group(P<0.05).(2)There were no significant differences in gene frequency and genotype frequency distribution of Rs4995894 and Rs4292355 between the 2 groups(P>0.05),and there were significant differences in the frequency distribution of the TRPC3 gene SNP site Rs2292232(P<0.05).The distribution frequency of Rs2292232 point TT genotype was significantly higher in the EH+LVH group than that of normal control group(P<0.05).(3)The Logistic regression analysis showed that woman(OR=0.332,95%CI:0.181-0.610,P<0.05),higher 24-hour DBP(OR=1.035,95%CI:1.014-1.056,P<0.05)and the TRPC3 gene SNP Rs2292232 TT genotype(OR=2.105,95%CI:1.109-3.995,P<0.05)were independent risk factors for LVH in hypertensive patients.Conclusion The Rs2292232 SNP of TRPC3 gene is risk factor for EH patients with LVH,and TT gene type is more likely to develop LVH.
作者
蒋永超
孙晓靖
陈玉岚
张俊仕
珠勒皮亚司马义
徐新娟
张向阳
JIANG Yong-chao;SUN Xiao-jing;CHEN Yu-lan;ZHANG Jun-shi;ZHU LEPIASimayi;XU Xin-juan;ZHANG Xiang-yang(Department of Hypertension,the First Teaching Hospital of Xinjiang Medical University,Urumqi 830054,China;Department of Critical Medicine,the Second Affiliated Hospital of Xinjiang Medical University)
出处
《天津医药》
CAS
北大核心
2020年第8期731-735,共5页
Tianjin Medical Journal
基金
新疆维吾尔自治区自然科学基金项目(2016D01C211)。
关键词
瞬时受体电位通道
多态性
单核苷酸
基因频率
原发性高血压
左心室肥厚
TRPC3
transient receptor potential channels
polymorphism
single nucleotide
gene frequency
essential hypertension
left ventricular hypertrophy
transient receptor potential-canonical 3 channels
