摘要
目的:探究早期跑轮联合针刺对重度脑外伤大鼠运动功能及前额叶皮质脑源性神经生长因子(BDNF)表达的影响。方法:50只SD大鼠随机分为假手术组、模型组、针刺组、跑轮组和联合组,每组10只。改良Feeney法制备重度脑外伤大鼠模型。术后第二天开始干预,然后在不同时间点利用改良神经功能缺损评分(mNSS)和足误试验分别评估大鼠神经功能与协调功能。2周后利用Western Blot和免疫组化分别检测皮质BDNF的表达和阳性反应。结果:治疗第7、10、14天联合组大鼠mNSS评分明显低于模型组(P<0.05),治疗第10、14天各干预组大鼠足误试验评分明显提高(P<0.05)。2周后,与假手术组比较,模型组前额叶皮质BDNF表达降低(P<0.05),而联合组BDNF的表达显著提高(P<0.05)。免疫组化染色结果显示,与模型组比较,针刺组和跑轮组BDNF的表达增加(P<0.05);而与针刺组和跑轮组比较,联合治疗组BDNF平均光密度值明显增加(P<0.05)。结论:早期跑轮联合针刺可能通过提高皮质中BDNF的表达来改善脑外伤大鼠的运动功能。
Objective:To investigate the effects of early wheel running combined with acupuncture on motor function and the expression of BDNF in prefrontal cortex of rats.Method:Fifty SD rats were randomly divided into five groups:sham group,model group,acupuncture group,running wheel group and combined group.The modified neurologic defect score(mNSS)and foot fault test were used to evaluate the neural function and coordination function of rats at different time points.Western Blot and Immunohistochemical was used to determine the expression of brain-derived neurotrophic factor(BDNF)and positive reaction in five groups.Result:The expression of BDNF in the prefrontal cortex of the model group was decreased(P<0.05),while the expression of BDNF was significantly increased in the combined group(P<0.05).The immunohistochemical staining results showed that compared with the model group,the expression of BDNF in the acupuncture group and the running wheel group increased(P<0.05);Compared with the acupuncture group and the running wheel group,the average light density of BDNF in the combined treatment group increased significantly(P<0.05).Conclusion:Early wheel running combined with acupuncture may improve the motor function of brain injury rats by increasing the expression of BDNF in the cortex.
作者
陈林
郑伸伸
杨翊
刘经星
张刚
袁也
张神怡
黄琴
CHEN Lin;ZHENG Shenshen;YANG Yi(Dept.of Rehabilitation,Renmin Hospital,Hubei University of Medicine,Shiyan,Hubei,442000;不详)
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2020年第7期794-800,共7页
Chinese Journal of Rehabilitation Medicine
基金
十堰市引导性项目(18Y53)。
关键词
脑外伤
运动功能
跑轮
针刺
traumatic brain injury
motor function
running wheel
acupuncture
