摘要
利用高通量测序技术对清香型大曲白酒含单宁高粱和不含单宁高粱酒醅中的细菌种群进行了分析,研究了高粱单宁含量对细菌种群的影响。分别采集发酵第1、3、7和21天的酒醅,提取细菌基因组DNA,并扩增了16S r DNA的V4区,扩增产物构建文库并进行高通量测序,测序结果进行生物信息学分析。结果表明,两种酒醅中的主要细菌种群有片球菌属(Pediococcus)、魏斯氏菌属(Weissella)、乳杆菌属(Lactobacillus)、芽孢杆菌属(Bacillus)、拟杆菌属(Bacteroides)、窄食单胞菌属(Stenotrophomonas)等。在发酵的第1天和第3天,不含单宁酒醅的细菌种群多样性高于含单宁酒醅的;但之后却完全相反。单宁含量对细菌种群差异的贡献率为22.39%,芽孢杆菌属(Bacillus)、肠球菌属(Enterococcus)、类芽孢杆菌属(Paenibacillus)与单宁含量呈显著负相关(P<0.05)。
The bacterial community of fermented sorghum grains with or without tannin were analyzed by high-throughput sequencing in Fen-flavor Daqu Baijiu(Chinese liquor), and the effects of tannin content of sorghum on bacterial community were studied. Fermented grains were collected at the1 st, 3 rd, 7 th and 21 st day of fermentation, the bacterial genomic DNA were extracted, V4 regions of 16 S r DNA were amplified, and the amplification products were used for library construction and then measured by high-throughput sequencing, the sequences achieved were analyzed by bioinformatics.The results showed that the major bacterial community was Pediococcus, Weissella, Lactobacillus, Bacillus, Bacteroides, Stenotrophomonas and so on in two kinds of fermented grains. The diversity indexes of fermented grains without tannin were higher than those of fermented grains with tannin at the 1 st and 3 rd day of fermentation;whereas afterwards the situation was completely opposite. The tannin content could attribute 22.39% differences to bacterial community, and three genera(Bacillus, Enterococcus and Paenibacillus) had significant negative correlations to the tannin content(P<0.05).
作者
杨玲
王琪
郭旭凯
段冰
郭睿
邵强
柳青山
YANG Ling;WANG Qi;GUO Xukai;DUAN Bing;GUO Rui;SHAO Qiang;LIU Qingshan(Institute of Sorghum,Shanxi Academy of Agricultural Sciences,Jinzhong 030600,China;School of Life Science,Shanxi University,Taiyuan 030006,China)
出处
《中国酿造》
CAS
北大核心
2020年第7期83-88,共6页
China Brewing
基金
国家现代农业产业技术体系(CARS-06-13.5-A30)
山西省青年科技研究基金(201701D221178)。
关键词
清香型大曲白酒
高粱
单宁
酒醅
细菌种群
高通量测序
Fen-flavor Daqu Baijiu
sorghum
tannin
fermented grains
bacterial community
high-throughput sequencing
