摘要
采用Box-Behnken响应面试验设计法对玫瑰茄总黄酮超声波-微波协同提取工艺进行优化,研究了乙醇浓度、液固比、提取时间、微波功率、提取次数5个单因素对总黄酮提取量的影响,建立了乙醇浓度、液固比和微波功率的三因素回归模型,并研究其抗氧化活性。结果表明,提取的最佳工艺参数为乙醇浓度40%、液固比50∶1(mL/g)、微波功率250 W、提取时间20 min、超声波功率50 W,在此条件下提取2次,总黄酮提取量为40.2 mg/g。同时以维生素C作对照,研究了玫瑰茄总黄酮粗提物和纯化物的体外抗氧化活性。结果表明,玫瑰茄总黄酮粗提物和纯化物都具有较强的抗氧化活性,能够有效清除O2^-·自由基和DPPH自由基,清除能力与浓度呈较明显的量效关系,但抗氧化能力较维生素C弱。
The ultrasonic-microwave assisted extraction of the flavonoids from Hibiscus sabdariffa L.was optimized by response surface analysis with a Box-Behnken design.The effects of five important factors,liquid-material ratio,ethanol concentraion,extraction time,microwave power and the extraction times,on the flavonoids extraction of H.sabdariffa Lwere investigated in the single factor experiments.The regression model of the liquid-material ratio,ethanol concentration and microwave power to solvent was established.The optimum technology condition for the ultrasonic-microwave assisted extraction of the flavonoids from H.sabdariffa L.was determined as follow:the ethanol concentration 40%,liquid-material ratio 50∶1(mL/g),microwave power 250 W,extraction time 20 min and ultrasonic power 50 W,extracted twice.Under the conditions,the flavonoids yield was 40.2 mg/g.Using vitamin C as the control,the antioxidant activity of the crude extract and purified flavonoids was studied.The crude extract and purified flavonoids from H.sabdariffa L.had a strong antioxidant activity,and they had an antioxidation effect on DPPH and O2^-·,and the scavenging activity had an obvious dose-effect relationship of concentration,but the antioxidant activity was weaker than that of vitamin C.
作者
黄琼
黄晓梅
HUANG Qiong;HUANG Xiaomei(Fujian Vocational College of Agriculture,Fuzhou,Fujian 350003,China)
出处
《热带作物学报》
CSCD
北大核心
2020年第6期1242-1250,共9页
Chinese Journal of Tropical Crops
基金
福建省教育厅中青年项目(No.JAT171003)。
关键词
响应面法
超声波-微波协同
玫瑰茄总黄酮
提取
抗氧化活性
response surface methodology
ultrasound-microwave assisted
total flavonoids from Hibiscus sabdariffa L.
extraction
antioxidation activity
