摘要
目的研究茵陈标准汤剂的量值传递规律。方法制备15批茵陈标准汤剂,HPLC法建立标准汤剂及其原料饮片指纹图谱,建立对照指纹图谱,标定共有峰,并进行相似度评价,四级杆飞行时间串联质谱对共有峰进行鉴定,对采用对照品比对确认的成分进行含量测定,通过共有峰传递数及峰面积比值变化、指标成分转移率、出膏率对标准汤剂进行量值传递规律分析。结果茵陈饮片和标准汤剂指纹图谱相似度均大于0.9,分别标定共有峰16个和15个,共有峰个数传递率93.75%,采用对照品比对确认了共有峰中的1-咖啡酰奎宁酸、新绿原酸、绿原酸、咖啡酸、隐绿原酸、对羟基苯乙酮、1,3-二咖啡酰奎宁酸、芦丁、金丝桃苷、异槲皮苷、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸和4,5-二咖啡酰奎宁酸13个成分,在茵陈中首次检测到了咖啡酰奎宁酸葡萄糖苷和黄酮二糖碳苷芹菜素-6,8-C-二葡萄糖苷,标准汤剂和饮片共有峰面积比值与成分转移率呈现较好的正向直线关系,新绿原酸、隐绿原酸、1,3-二咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸平均转移率超过100%,绿原酸、3,5-二咖啡酰奎宁酸平均转移率分别只有47.59%和22.33%,提示标准汤剂制备过程中有机酸类成分可能发生了相互转化,绿原酸可能部分转化成了隐绿原酸和新绿原酸,3,5-二咖啡酰奎宁酸可能部分转化成了1,3-二咖啡酰奎宁酸和3,4-二咖啡酰奎宁酸,芦丁、金丝桃苷平均转移率分别为31.36%、28.36%,其余成分平均转移率在50%~70%,标准汤剂平均出膏率为19.76%。结论研究揭示的茵陈标准汤剂量值传递规律为茵陈配方颗粒及含茵陈经典名方物质基准建立奠定了基础。
Objective To study the law of quality value transmitting of Artemisiae Scopariae Herba(ASH) standard decoction. Methods Fifteen batches of ASH standard decoction were prepared. Fingerprints of these 15 batches standard decoction and its raw pieces were determined by HPLC, the control fingerprints were established, the common peaks were calibrated and the similarity was evaluated. Components of common peaks were identified by Q-TOF/MS and the contents of components confirmed by reference substance were determined. The common peaks transfer number, peak area ratio, index components transfer rate and extraction rate were used to analyze the quality value transfer rule of standard decoction. Results The similarity of fingerprints of ASH and its standard decoction were greater than 0.9, 16 and 15 common peaks were calibrated respectively, and the transfer rate of the common peaks number was 93.75%. Thirteen components of the common peaks were confirmed by reference substance, including 1-caffeoylquinic acid, neochlorogenic acid, chlorogenic acid, caffeic acid, cryptochlorogenic acid, p-hydroxyacetophenone, 1,3-dicaffeoylquinic acid, rutin, hyperoside, isoquercetin, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid. Caffeoylquinic acid glucoside and a di-C-glycoside named apigenin 6,8-di-C-β-D-glucoside were first detected in ASH. The ratio of the common peaks area of ASH standard decoction to that of ASH raw pieces showed a good positive linear relationship with the components transfer rate. The average transfer rates of neochlorogenic acid, cryptochlorogenic acid, 1,3-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid were more than 100%, while the average transfer rates of chlorogenic acid and 3,5-dicaffeoylquinic acid were only 47.59% and 22.33% respectively, suggesting that the organic acid components may be transformed into each other during the preparation of standard decoction, and part of chlorogenic acid may be transformed into cryptochlorogenic acid and neochlorogenic acid;3,5-dicaffeoylquinic acid may be partially converted into 1,3-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid. The average transfer rates of rutin and hyperoside were 31.36% and 28.36%, respectively, and that of other components were between 50% and 70%. The average extraction rate of standard decoction was 19.76%. Conclusion The laws of quality value transmitting of ASH standard decoction revealed in this study laid a foundation for the establishment of the material reference of ASH formula granules and the classic prescriptions containing ASH.
作者
倪丽丽
戴莹
窦志华
王兆龙
周云中
NI Li-li;DAI Ying;DOU Zhi-hua;WANG Zhao-long;ZHOU Yun-zhong(Nanjing University of Chinese Medicine,Nanjing 210023,China;Nantong Clinical Medical College of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nantong 226006,China;Jinghua Pharmaceutical Group Co.,Ltd.,Nantong 226005,China)
出处
《中草药》
CAS
CSCD
北大核心
2020年第11期2954-2966,共13页
Chinese Traditional and Herbal Drugs
基金
江苏省重点研发计划(社会发展)项目(BE2018674)
江苏省中医药科技计划项目(YB201836)
南通市“226工程”科研资助计划项目(201714)。
关键词
茵陈
标准汤剂
物质基准
量值传递
指纹图谱
转移率
出膏率
新绿原酸
隐绿原酸
1
3-二咖啡酰奎宁酸
3
4-二咖啡酰奎宁酸
绿原酸
3
5-二咖啡酰奎宁酸
Artemisiae Scopariae Herba
standard decoction
material reference
quality value transmitting
fingerprint
transfer rate
extraction rate
neochlorogenic acid
cryptochlorogenic acid
1
3-dicaffeoylquinic acid
3
4-dicaffeoylquinic acid
chlorogenic acid
3
5-dicaffeoylquinic acid
