摘要
目的分析活动性类风湿关节炎(RA)患者Toll样受体9(TLR9)在单核细胞亚群中的表达及单核细胞经配体活化后分泌细胞因子的功能,初步探讨外周血单核细胞TLR9在RA疾病中的作用。方法收集32例活动性RA患者(RA组)和32例健康人对照(HC组)外周血,流式细胞术分析单核细胞亚群TLR9表达;体外培养单核细胞,经TLR9配体CpG-2006活化培养48 h,Luminex 200液相芯片技术检测培养上清液白细胞介素(IL)-6、肿瘤坏死因子(TNF)-α和单核细胞趋化蛋白(MCP)-1浓度。结果与HC组相比,RA患者外周血3个单核亚群TLR9平均荧光强度(MFI)均显著上升(CD14++CD16-:211.4±35.3 vs 73.4±11.3;CD14+CD16+:214.9±32.6 vs 69.3±14.1;CD14lowCD16+:141.8±22.6 vs 12.8±4.8),差异均有统计学意义(P<0.05)。与HC组活化后相比,RA组活化后上清液细胞因子IL-6[(209.8±32.9)pg/mL vs(71.1±12.6)pg/mL]、TNF-α[(264.3±35.4)pg/mL vs(76.2±18.7)pg/mL]、MCP-1[(167.6±19.2)pg/mL vs(23.3±7.4)pg/mL]浓度均显著上升,差异均有统计学意义(P<0.05)。结论活动性RA患者外周血单核细胞亚群高表达TLR9,促进炎性细胞因子的分泌,在RA疾病过程中发挥重要的作用。
Objective To explore the role of Toll-like receptor 9(TLR9)on peripheral monocytes in the pathogenesis of rheumatoid arthritis(RA)by assessing the expression levels of TLR9 on different monocyte subsets of active RA patients and their cytokines secretion in the monocytes with response to TLR9 agonists.Methods A total of 32 active RA patients and 32 healthy controls were enrolled in this study.The levels of TLR9 on different subsets of monocyte in peripheral blood were evaluated by flow cytometry.The enriched monocytes were cultured in vitro and stimulated with TLR9 agonists CpG-2006 for 48 h.The concentrations of cytokines IL-6,tumor necrosis factor-α(TNF-α)and monocyte chemotactic protein 1(MCP-1)in the supernatant were determined by Luminex 200 liquid chip.Results Compared with the healthy controls,the mean fluorescence intensity(MFI)of TLR9 were significantly increased on all the three subsets of monocytes(211.4±35.3 vs 73.4±11.3 in CD14++CD16-subset;214.9±32.6 vs 69.3±14.1 in CD14+CD16+subset and 141.8±22.6 vs 12.8±4.8 in CD14lowCD16+subset)and there were significantly difference(P<0.05).Compared with the healthy controls,the concentrations of cytokines IL-6,TNF-αand MCP-1 in the supernatant of RA patients were significantly increased after stimulation with TLR9 agonists[(209.8±32.9)pg/mL vs(71.1±12.6)pg/mL,(264.3±35.4)pg/mL vs(76.2±18.7)pg/mL,(167.6±19.2)pg/mL vs(23.3±7.4)pg/mL],and there were significantly difference(P<0.05).Conclusion The different subsets of peripheral blood monocyte from the patients with active RA showed increased TLR9 expression which may promote higher production of inflammatory cytokines and play important roles in the pathogenesis of RA.
作者
王绪山
王敏
李静
陈开勇
WANG Xushan;WANG Min;LI Jing;CHEN Kaiyong(Department of Clinical Laboratory, Guanyun County People′s Hospital, Guanyun 222200, Jiangsu, China)
出处
《临床检验杂志》
CAS
2020年第4期263-266,共4页
Chinese Journal of Clinical Laboratory Science
