摘要
为研究表面活性剂P188是否对大鼠自体脂肪移植后的炎症浸润具有抑制作用。建立大鼠自体脂肪移植动物模型,分为对照组(生理盐水组)和实验组(P188组),于1、2、4周取出脂肪组织,测定体质量和体积;通过HE染色观察移植后脂肪炎症浸润的程度;通过RT-PCR和ELISA检测相关炎症因子基因和蛋白水平的表达。脂肪移植4周后,对照组与实验组的脂肪体质量、体积具有差异性[体质量:(0.13±0.02) g vs(0.19±0.02) g,P<0.01;体积:(0.15±0.03) ml vs(0.19±0.01) ml,P<0.05];HE结果显示,移植4周后实验组炎症浸润面积低于对照组;RT-PCR和ELISA结果显示P188抑制IL-1β、IL-6、TNF-α的mRNA以及蛋白表达。实验结果表明P188对大鼠自体脂肪移植后的炎症浸润具有抑制作用。
To study whether Kolliphor P188 could inhibit the inflammation during autologous fat transplantation,from the perspective of inflammation to analyze the effect of P188 on transplantation. The animal models of autologous fat transplantation were divided into the control group(normal saline group) and the experimental group(P188 group). Weight and volume were measured after fat tissue was removed at time points 1, 2 and 4 weeks. The degree of fat inflammatory infiltration after transplantation was observed by HE staining. Finally, the mRNA and protein levels of inflammation-related factors IL-1α,1 L-1β,IL-6,TNF-α were detected by RT-PCR and ELISA. After 4 weeks of fat transplantation, there were differences in fat weight and volume between the control group and the experimental group [weight:(0.13±0.02) g vs(0.19±0.02) g, P<0.01, volume:(0.15±0.03) ml vs(0.19±0.01) ml, P<0.05]. HE staining results showed that the inflammatory infiltration area of P188 group was lower than that of normal saline group 4 weeks later. RT-PCR and ELISA results showed that 4 weeks after fat transplantation, P188 inhibited the mRNA levels of inflammatory cytokines IL-1α,1 L-1β,IL-6,TNF-α and the protein expressions of IL-1α,1 L-1β,IL-6,TNF-α. It’s proved that P188 can inhibit inflammatory infiltration after autologous fat transplantation in rats.
作者
李吟秋
倪子樵
王鑫
宋家骞
朱飞
Li Yinqiu;Ni Ziqiao;Wang Xin(Dept of Plastic Surgery,The First Affiliated Hospital of Anhui Medical University,Hefei,230022)
出处
《安徽医科大学学报》
CAS
北大核心
2020年第4期629-633,639,共6页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:30973124)。
