摘要
目的探讨桂西地区壮族不育男性畸形精子症的特点。方法收集2019年1月至8月就诊的2275例桂西壮族男性不育患者,采用Diff-Quik染色对精子形态进行研究;使用计算机辅助精液分析(CASA)技术检测精子前向运动力、浓度、总活力等;采用固相BAPNA法分析精子顶体酶活性。对比畸形和正常精子患者的精液常规参数、顶体酶活性。分析精子正常形态百分率与精液常规参数、精子顶体酶活性的相关性。结果精液形态检测发现畸形精子症患者占47.47%,在畸形精子症患者中,中度畸形占49.35%,比重最大;在精子畸形部位中,以头部畸形为主,占69.83%;在精子头部畸形类型中,锥形头比例最高,占48.00%。畸形组患者的精子前向运动力、浓度、总活力、顶体酶活性均低于正常组,差异有统计学意义(P均<0.01)。精子正常形态百分率与精子前向运动力、浓度、总活力、顶体酶活性呈正相关(P均<0.01)。结论桂西地区壮族不育男性人群中患畸形精子症较多,其中中度畸形比例最大,精子畸形部位以头部异常为主,在头部异常类型中锥形头常见。精子正常形态百分率与精子前向运动力、浓度、总活力、顶体酶活性呈正向关联。
Objective To investigate the characteristics of infertile men with teratozoospermia of Zhuang nationality in western Guangxi.Methods From January to August 2019,2275 male infertile patients of Zhuang nationality in western Guangxi were collected.Diff-Quik staining was used to study the morphology of sperm.Computer aided semen analysis(CASA) technology was used to test forward motility,concentration and total motility of sperm.And solid phase BAPNA method was used to analyze sperm acrosin activity.The semen routine parameters and acrosin activity of abnormal and normal spermatozoa were compared.And the correlation between the percentage of normal sperm morphology and the normal semen parameters and acrosin activity was analyzed.Results Semen morphology test showed that patients with teratozoospermia accounted for 47.47%,and among them,moderate teratozoospermia accounted for 49.35%,which had the highest proportion.Head deformity was the main part of sperm deformity,accounting for 69.83%,and in the type of sperm head deformity,the proportion of cone-shaped head was the highest,accounting for 48.00%.The sperm forward motility,concentration,total activity and acrosin activity in the abnormal sperm group were lower than those in the normal sperm group,difference was statistically significant(all P<0.01).There was a positive correlation between the percentage of normal sperm morphology and sperm forward motility,concentration,total activity and acrosin activity(all P<0.01).Conclusion There are many abnormal spermatozoa in the infertile male population of Zhuang nationality in western Guangxi,among which the proportion of moderate deformity is the largest,and the abnormal position of spermatozoa is mainly head abnormality,and cone-shaped head is common in the type of head abnormality.The percentage of normal sperm morphology is positively correlated with forward motility,concentration,total activity and acrosin activity.
作者
刘中林
杨凤莲
韦玉霞
庞晓霞
廖碧云
陈兴鸿
王俊利
LIU Zhonglin;YANG Fenglian;WEI Yuxia;PANG Xiaoxia;LIAO Biyun;CHEN Xinghong;WANG Junli(Center of Reproductive Medicine of Affiliated Hospital,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;School of Pharmacy,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Graduate School,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)
出处
《右江医学》
2020年第4期296-299,共4页
Chinese Youjiang Medical Journal
基金
广西自然科学基金(2018GXNSFAA050034,2019GXNSFBA245034)
广西教育厅高校中青年教师基础能力提升项目(2018KY0437)。
关键词
男性不育
精液质量
精液参数
桂西
壮族
male infertility
semen quality
semen parameters
western Guangxi
Zhuang nationality
