摘要
大豆胞囊线虫病(Soybean cyst nematode,Heterodera glycines Ichinohe)是一种严重影响大豆产量的病害,我国东北地区以3号生理小种(SCN Race 3)为主。QTL定位是挖掘抗性基因最有效的方法之一,与SCN相关的QTL报道较多,其由不同遗传群体获得,但在不同遗传群体间较难重复。研究采用图谱整合软件BioMercatorV4.2挖掘SCN Race 3抗性基因。将收集整理的10篇文献发表的33个QTL整合在遗传图谱GmComposite2003上,通过元分析方法得到一致性QTL,最终获得3个抗性候选区段,发现18个SCN抗性基因,其中17个PK型基因和1个LRR型基因,分别位于7、15和18染色体中。对潜在SCN抗性基因分析蛋白结构域发现,17个PK型基因分别含有丝/苏氨酸蛋白激酶活性、钙依赖蛋白激酶活性、分裂原活化蛋白激酶活性、半胱氨酸蛋白激酶和组/精/赖氨酸蛋白激酶活性。这些基因编码蛋白结构域与植物抗病相关,结果可为抗SCN基因挖掘和选育抗SCN品种(系)提供理论基础。
Soybean cyst nematode(SCN,Heterodera glycines Ichinohe)is a serious disease affecting soybean yield.SCN Race 3 is dominant in northeast China.QTL mapping is one of the most effective methods for mining resistance genes;however,these QTLs were obtained from different genetic populations and are difficult to repeat among different genetic populations.In this study,map integration software BioMercatorV4.2 was used to mine SCN Race 3 resistance genes.The total 33 QTL published in 10 articles were integrated into the genetic map GmComposite2003.Additionally,the consistent QTLs were obtained by meta-analysis,and finally three resistance candidate segments were obtained,and 18 SCN resistance genes,17 PK type genes and 1 LRR type gene were found,which were located in 7,15 and 18 chromosomes.Protein domain analysis of potential SCN resistance genes showed that 17 PK genes contained serine/threonine protein kinase activity,calcium dependent protein kinase activity,mitogen activated protein kinase activity,cysteine protein kinase activity and group/arginine protein kinase activity.Combined with the previous research results,it was shown that the domain of proteins encoded by these genes was related to plant disease resistance,which provided a theoretical basis for SCN-resistant gene mining and breeding of SCN-resistant varieties(lines).
作者
韩英鹏
田利峥
姜海鹏
包冬芳
王俊
赵雪
HAN Yingpeng;TIAN Lizheng;JIANG Haipeng;BAO Dongfang;WANG Jun;ZHAO Xue(Institute of Soybean Research,Key Laboratory of Soybean Biology in Chinese of Ministry Education,Key Laboratory of Soybean Biology and Breeding(Genetics)of Ministry of Agriculture and Rural Affairs,Northeast Agricultural University,Harbin 150030,China)
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2020年第4期10-17,共8页
Journal of Northeast Agricultural University
基金
黑龙江省杰出青年基金项目(JC2018007)
国家自然科学基金面上项目(31671717,31971967)
国家科技重大专项和重点研发项目(课题)省级资助项目(GX17B002)
黑龙江省博士后项目(LBH-Q17015)
国家重点研发计划项目(2016YFD0100304)。
