摘要
目的:观察手针浅刺和手针深刺"内关"穴对氯化钡诱发的心律失常家兔心肌组织形态和心肌中缝隙连接蛋白43(Cx43)表达的影响。方法:新西兰兔随机分为生理盐水组、模型组、浅刺组和深刺组,每组15只。氯化钡耳缘静脉推注复制心律失常家兔模型。浅刺组直刺家兔右侧"内关"穴,进针深度为3 mm,行震颤催气手法10 min;深刺组进针深度为5~8 mm,针尖触碰到正中神经使家兔右上肢抽动3次后,留针10 min。记录心律失常起始时间和持续时间,HE染色法观察心肌组织形态,免疫组织化学法检测心室肌中Cx43表达。结果:与模型组比较,两针刺组心律失常起始时间均延长(P<0.01),心律失常持续时间均明显缩短(P<0.01)。HE染色显示模型组家兔心肌纤维排列紊乱,出现波浪样变,心肌间质有浆液、红细胞渗出;两针刺组均有不同程度的改善。免疫组织化学检测显示模型组家兔心肌中Cx43分布紊乱,在闰盘处几乎无明显的条带状分布;深刺组Cx43大部分呈条带状分布于闰盘处;浅刺组Cx43呈条带状、团状、点状,在端-端连接和侧-侧连接处均可见。与生理盐水组比较,模型组心室肌中Cx43表达显著减少(P<0.01);深刺组和浅刺组较模型组Cx43表达显著增加(P<0.01),且深刺组Cx43表达高于浅刺组(P<0.01)。结论:"内关"穴浅刺和深刺均可以显著减少氯化钡诱发的心律失常持续时间,改善家兔心肌组织的病理改变和Cx43表达减少、分布紊乱的情况,提示除正中神经外,"内关"浅层也有参与传递针刺信息的途径。
Objective To compare the therapeutic effect of shallow and deep needling at "Neiguan"(PC6) in the treatment of arrhythmia in rabbits. Methods Male New Zealand rabbits were randomly divided into saline(n=15), model(n=12), shallow needling(n=13) and deep needling(n=12) groups. The arrhythmia model was established by ear intravenous injection of Barium chloride(0.4%, 1 mL/kg). Acupuncture needle was inserted into the superficial fascia(about 3 mm beneath the skin) or deep layer(5 to 8 mm, near the median nerve) of local PC6 tissue(fore limb), manipulated for a while and then retained for 10 min. Histopathological changes of myocardium was observed after H.E. stain, and the immunoactivity of connexin 43(Cx43) detected by immunohistochemistry(IHC). Results Various degrees of arrhythmia and down-regulated expression of myocardial Cx43 were observed in all rabbits after modeling. After EA intervention and compared with the model group, the initial time of arrhythmia and Cx43 expression were obviously increased(P<0.01), and the duration of arrhythmia was significantly shortened in both deep and shallow needling groups(P<0.01). Compared with the shallow needling group, the Cx43 expression was increased in the deep needling group(P<0.01). H.E. staining showed disordered and wavy arrangement of myocardial fibers, with exudation of serous and erythrocytes in the myocardial interstitium in the model group, which was relatively mild in both needling groups. IHC showed disordered distribution of Cx43 in the ventricular myocytes and almost no obvious band-like distribution at the discs in rabbits of the model group, and abundant distribution of Cx43 in the sacral disc in the deep needling group, and strip-shaped, cluster-like, point-like, visible at both end-to-end connections and side-to-side connections in the shallow needling group.Conclusion Both shallow and deep needling can significantly reduce the duration of arrhythmia in arrhythmia rabbits, which may be associated with its effect in up-regulating expression of myocardial Cx43 protein.
作者
林怡
彭超
杨守亲
古春凌
葛东宇
董瑞娟
程凯
LIN Yi;PENG Chao;YANG Shou-qin;GU Chun-ling;GE Dong-yu;DONG Rui-juan;CHENG Kai(School of Acupuncture-moxibustion and Massage,Beijing University of Chinese Medicine,Beijing 100029,China;College of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《针刺研究》
CAS
CSCD
北大核心
2020年第4期269-274,共6页
Acupuncture Research
