摘要
为了确定大口黑鲈成鱼适合的麻醉方式和麻醉剂量,研究了不同浓度的丁香酚和MS-222对大口黑鲈的静水麻醉效果。在最适麻醉质量浓度下模拟运输24 h,记录24 h存活率并测定麻醉0.5、4、8、16和24 h后的血清生化指标、水质p H、氨氮以及麻醉剂的残留量。结果表明:随着丁香酚和MS-222浓度增加,入麻时间缩短,复苏时间延长;丁香酚和MS-222的最适质量浓度分别为18~20 mg/L、50~60 mg/L时,两麻醉组模拟运输和复苏24 h后的成活率为100%,显著高于无麻醉运输组的80%和90%(p<0.05);随着麻醉时间延长,丁香酚和MS-222组血清中的皮质醇(COR)分别从336.83pg/m L下降到291.41pg/m L,从286.11上升到367.05pg/m L(p<0.05),丁香酚组血清中的COR显著低于对照组;葡萄糖分别从14.36、7.48 mg/dL下降到7.36、3.11 mg/dL(p<0.05),而丁香酚组中乳酸脱氢酶(LDH)、碱性磷酸酶(AKP)、谷草转氨酶(GOT)、尿素氮(BUN)的浓度显著低于MS-222组(p<0.05);残留检测结果表明在麻醉0.5h时,鱼肉中丁香酚含量达到最大值5.14mg/kg,在清水中复苏1d时丁香酚残留量为0.45mg/kg;而MS-222组在8 h残留量达到最大值为36.24 mg/kg,复苏4 d时基本消除完全。结果显示,丁香酚剂量小,代谢快,在诱发氧化应激和组织损伤时有较少的副作用。
To determine the appropriate anesthetic method and dose for perch, hydrostatic anesthesia effects of two common fish anesthetics(MS-222 and eugenol) on perch under different concentrations was compared. An experiment of 24-hour anesthesia transport was carry out under the optimal anesthetic concentrations of MS-222 and eugenol. The changes of serum biochemical indexes, water quality pH, ammonia nitrogen and residual concentration of anesthetics were measured after 0.5, 4, 8, 16 and 24 h of anesthesia. The results showed that the anesthetic time was shortened and the recovery time was prolonged with the increase of anesthetics concentration. The concentrations of eugenol and MS-222 are suitable for long-distance transportation of perch at 18-20 mg/L and 50-60 mg/L respectively. The survival rate during the transportation and after 24 h of recovery for the anesthesia group were both 100%, there were significantly higher than those for the non-anesthetic transportation group(p<0.05). The serum cortisol(COR) in eugenol group and MS-222 group decreased from 336.83 pg/m L to 291.41 pg/m L and increased from 286.11 to 367.05 pg/m L(p<0.05) with the prolongation of anesthesia time, and the serum COR in eugenol group was significantly lower than that in control group. The glucose decreased from 14.36, 7.48 mg/d L to 7.36, 3.11 mg/d L(p<0.05), respectively. The concentrations of lactate dehydrogenase(LDH), alkaline phosphatase(AKP), glutamic oxaloacetic transaminase(GOT), urea nitrogen(BUN) in eugenol group were significantly lower than those in MS-222 group(p<0.05). Residual test results showed that the maximum eugenol content was 5.14 mg/kg at 0.5 h of anesthesia and the residual amount of eugenol was 0.45 mg/kg at 1 day of resuscitation in clear water. In MS-222 group, the maximum content was 36.24 mg/kg after 8 h of anesthesia, and it was almost eliminated after 4 days of resuscitation. The results showed that eugenol had low dosage, fast metabolism and fewer side effects in inducing oxidative stress and tissue damage.
作者
李宁
白婵
熊光权
李瑞
王彩霞
张金木
任红佳
王炬光
廖涛
LI Ning;BAI Chan;XIONG Guang-quan;LI Rui;WANG Cai-xia;ZHANG Jin-mu;REN Hong-jia;WANG Ju-guang;LIAO Tao(Institute of Agricultural Product Processing and Nuclear Agriculture Technology,Wuhan 430064,China;College of Fisheries Huazhong Agricultural University,Wuhan 430070,China;School of Chemical and Environmental Engineering,Wuhan Engineering University,Wuhan 430205,China)
出处
《现代食品科技》
EI
CAS
北大核心
2020年第3期56-64,71,共10页
Modern Food Science and Technology
基金
现代农业产业技术体系专项资金资助(CARS-46)
湖北省科技特派员项目
湖北省农业科技创新中心项目(2019-620-000-001-25)。
关键词
大口黑鲈
丁香酚
三卡因甲磺酸盐
生理生化指标
代谢残留
large mouth bass
eugenol
tricaine mesylate
physiological and biochemical indicators
residual metabolism
