摘要
目的制备秋水仙碱海藻酸钠微球并研究其表征。方法采用油包水(W/O)型机械乳化离子交联法制备微球,通过单因素实验确定显著影响微球成球性的因素,考察海藻酸钠质量浓度、均质机转速和机械乳化时间对微球粒径的影响,并检测微球体外黏膜黏附、载药及体外释放特性。结果海藻酸钠质量浓度、均质机转速和机械乳化时间均不同程度影响微球粒径及微球粒径集中趋势。以海藻酸钠质量浓度为20 mg·mL^-1、转速为2000 r·min^-1、时间为5 min制备微球,微球分散性良好,微球在质量浓度为20~50 mg·mL^-1时黏附性能良好,6 h内表现持续的药物释放特性,载药量为10.67%。结论该方法制备微球工艺稳定,体外黏附及释药性能良好。
Objective To prepare colchicine sodium alginate microspheres and to study their characteristics.Methods The microspheres were prepared by oil in water(W/O)mechanical emulsification cross-linking method.The factors affecting the sphericity of the microspheres were determined by single factor experiments.The effects of sodium alginate concentration,homogenizer speed and mechanical emulsification time on the particle size of the microspheres were investigated,and the mucosal adhesion,drug loading and in vitro release characteristics of microspheres were detected.Results The sodium alginate concentration,homogenizer speed and mechanical emulsification time all affected the particle size and concentration of microspheres in varying degrees.The microspheres prepared by sodium alginate concentration of 20 mg·mL^-1,rotating speed of 2000 r·min^-1 and time of 5 min were dispersed well.The drug loading was 10.67%and adhered to the mucosa at the concentration of 20-50 mg·mL^-1.Within 6 h,sustained drug release was observed.Conclusion The optimized technigue presents good stability and obvious effect of adhesion and drug release.
作者
高艳春
施文荣
涂春香
刘美艳
许炜钊
GAO Yanchun;SHI Wenrong;TU Chunxiang;LIU Meiyan;XU Weizhao(Academmy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;College of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)
出处
《西北药学杂志》
CAS
2020年第2期239-243,共5页
Northwest Pharmaceutical Journal
基金
福建省自然科学基金项目(编号:2016J01390)。
关键词
秋水仙碱
微球
W/O型机械乳化离子交联法
黏膜黏附
colchicine
microspheres
W/O mechanical emulsification cross-linking method
bioadhesion
