摘要
【目的】测定黄芩原药材、药渣中黄芩苷含量,并探讨其体外抗菌作用。【方法】采用紫外-分光光度法测定黄芩原药材、药渣1(复方提取)、黄芩药渣2(单方提取)的黄芩苷含量,二倍稀释法、纸片法分别测定黄芩原药材、黄芩药渣1、黄芩药渣2对副溶血弧菌、大肠杆菌、肺炎克雷伯菌的最低抑菌浓度、抑菌圈直径。【结果】黄芩原药材、黄芩药渣1、黄芩药渣2的黄芩苷含量分别为4.91%、2.05%、2.25%;黄芩原药材对副溶血弧菌、大肠杆菌、肺炎克雷伯菌的最低抑菌浓度分别为0.98、3.91、62.5 mg/mL,抑菌圈直径分别为17.5、15.5、9.0 mm;黄芩药渣1对副溶血弧菌、大肠杆菌、肺炎克雷伯菌的最低抑菌浓度分别为31.25、125、>250 mg/mL,抑菌圈直径分别为11.0、0、0 mm;黄芩药渣2对副溶血弧菌、大肠杆菌、肺炎克雷伯菌的最低抑菌浓度分别为31.25、>250、>250 mg/mL,抑菌圈直径分别为10.0、0、0 mm;阳性对照药中药复方NZ对副溶血弧菌、大肠杆菌、肺炎克雷伯菌的最低抑菌浓度分别为0.24、1.95、31.25 mg/mL,抑菌圈直径分别为22.0、22.0、21.0 mm。【结论】复方和单方提取黄芩药渣中黄芩苷含量及MIC值相差不大,以黄芩苷含量为指标来看,提取后的黄芩药渣仍有很好的利用价值。复方和单方黄芩药渣对不同菌株的MIC差异显著不同,与原药材相比有39倍差距之大。
Objective To determine the content of baicalin from Radix Scutellariae herbal materials and decoction residues,and to explore the in vitro antibacterial effect of baicalin.Methods UV-spectrophotometry was used to determine the content of baicalin from herbal materials,decoction residues 1(compounds extracting),and decoction residues 2(single herb extracting)of Radix Scutellariae.The double dilution method and paper method were used to determine the minimum inhibitory concentration(MIC)of herbal materials,decoction residues 1,and decoction residues 2 of Radix Scutellariae against the Vibrio parahaemolyticus,Escherichia coli,Klebsiella pneumoniae,and their diameter of the inhibition zone.Results The content of baicalin from herbal materials,decoction residues 1,and decoction residues 2 of Radix Scutellariae was 4.91%,2.05%,2.25%,respectively;the minimum inhibitory concentration of Radix Scutellariae herbal materials against Vibrio parahaemolyticus,Escherichia coli and Klebsiella pneumoniae was 0.98,3.91,62.5 mg/mL,and the diameter of the inhibition zone was 17.5,15.5,9.0 mm,respectively;the minimum inhibitory concentration of Radix Scutellariae decoction residues 1 against Vibrio parahaemolyticus,Escherichia coli and Klebsiella pneumoniae was 31.25,125,>250 mg/mL,and the diameter of the inhibition zone was 11.0,0,0 mm,respectively;the minimum inhibitory concentration of Radix Scutellariae decoction residues 2 against Vibrio parahaemolyticus,Escherichia coli and Klebsiella pneumoniae were 31.25,>250,>250 mg/mL,and the diameter of the inhibition zone was 10.0,0,0 mm,respectively;the minimum inhibitory concentration of the positive control drug Chinese herbal compound NZ against Vibrio parahaemolyticus,Escherichia coli and Klebsiella pneumoniae was 0.24,1.95,31.25 mg/mL,and the inhibition zone diameter was 22.0,22.0,21.0 mm,respectively.Conclusion The content and MIC value of baicalin between from Radix Scutellariae compounds extracting decoction residues and from Radix Scutellariae single herb extracting decoction residues are not significantly different,and the decoction residues have better utilization value with baicalin content as the index.The MIC values of the two decoction residues against different strains are significantly different,and the difference reaches 39 times that of the original herb.
作者
戴碧鑫
董碧莲
蔡延渠
朱盛山
DAI Bi-Xin;DONG Bi-Lian;CAI Yan-Qu;ZHU Sheng-Shan(Guangzhou Baiyunshan Xingzhu Pharmaceutical Co.,Ltd.,Guangzhou 510931 Guangdong,China;The Center for Drug Research and Development,Guangdong Pharmaceutical University,Guangzhou 510006 Guangdong,China)
出处
《广州中医药大学学报》
CAS
2020年第4期722-725,共4页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家自然基金青年科学基金资助项目(编号:81503260)
国家自然科学基金面上项目(编号:81373981)
广东省教育厅高校重点实验室滚动支持项目(编号:2013CXZDA021)。
关键词
黄芩
药渣
黄芩苷
体外抗菌
Radix Scutellariae
decoction residues
baicalin
In vitro antisepsis
