摘要
为了开发利用白酒大曲中的酵母菌资源,采用稀释涂布平板法,从芝麻香型白酒大曲中分离得到15株酵母菌株。利用26S rRNA基因序列分析技术对其进行分类鉴定。结果表明,其中6株酵母菌为酿酒酵母(Saccharomyces cerevisiae),6株为库德里阿兹威氏毕赤酵母(Pichia kudriavzevii),3株为热带假丝酵母(Candida tropicalis);并对代表菌株Y1、Y2及Y4进行了形态观察;最后通过随机扩增多态性DNA标记(random amplified polymorphic DNA,RAPD)对分离得到的酵母菌进行分型,表明6株酿酒酵母分属于5个株型,6株库德里阿兹威氏毕赤酵母分属于5个株型,3株热带假丝酵母分属于3个株型。
In order to explore and utilize the Daqu yeast resources,15 yeast strains were isolated from the sesame⁃fla⁃vor liquor Daqu by the dilution coating plate method,and identified by the 26S rRNA gene sequence analysis.The results showed that 6 yeast strains were Saccharomyces cerevisiae,6 strains were Pichia kudriavzevii,and 3 yeast strains were Candida tropicalis.The morphological observations of the representative strains Y1,Y2 and Y4 in the classification re⁃sults were carried out.The isolated yeasts were classified by random amplified polymorphic DNA(RAPD),which indi⁃cated that 6 strains of Saccharomyces cerevisiae belonged to 5 plant types,6 strains of Pichia cerevisiae belonged to 5 plant types,and 3 strains of Candida tropicalis belonged to 3 plant types.
作者
王志山
刘晓昆
陈文浩
许玲
刘洋
WANG Zhishan;LIU Xiaokun;CHEN Wenhao;XU Ling;LIU Yang(Solid⁃state Fermentation Resource Utilization Key Laboratory of Sichuan Province,Yibin University,Yibin 644000,Sichuan,China;College of Chemistry and Biological Engineering,University of Science and Technology Beijing,Beijing 100083,China;Shandong Bandaojing Ltd Co,Gaoqing 256300,Shandong,China)
出处
《生物资源》
CAS
2019年第6期550-556,共7页
Biotic Resources
基金
固态发酵资源利用四川省重点实验室开放基金项目(2018GTY008)
中央高校基本科研业务费资助项目(FRF-TP-18-012A1
FRF-BR-18-009B)
关键词
芝麻香型白酒
大曲
酵母
分离鉴定
sesame⁃flavored Baijiu
Daqu
yeast
isolation and identification
