摘要
[目的]探寻植物化感自毒化合物降解菌,更好地防治作物连作障碍。[方法]利用化感物质香草酸为唯一碳源,通过平板分离技术和液体培养高效液相色谱技术从花生土壤中分离筛选出1株降解香草酸的目的菌株V-5。对V-5进行底物降解范围的检测,并对其进行形态、生化特征和16S rRNA基因分子系统学研究。[结果]V-5菌株可以降解多种酚酸,其降解率分别为香草酸99.93%、对羟基苯甲酸99.85%、肉桂酸17.44%、丁香酸90.04%、苯甲酸98.69%、阿魏酸38.89%;该菌株为革兰氏阴性短杆菌,能还原硝酸盐为亚硝酸盐,水解精氨酸,同化葡萄糖、甘露糖、葡萄糖酸盐、癸酸、苹果酸、柠檬酸和苯乙酸,细胞色素氧化酶阳性,与恶臭假单胞菌(Pseudomonas putida)非常相似。16S rRNA基因系统发育分析表明V-5菌株与恶臭假单胞菌亲缘关系最近,序列相似度高达99%以上,因此鉴定所分离的酚酸降解菌V-5为恶臭假单胞菌。[结论]该研究为深入开发应用植物化感物质降解菌,促进农业可持续发展提供了理论依据。
[Objective]To explore the degradation bacteria of plant allelochemicals and autotoxic compounds and better control crop continuous cropping obstacles.[Method]Using the allelochemical vanillic acid as the sole carbon source,a strain V-5 which degraded vanillic acid was isolated and screened from peanut soil by plate separation technique and liquid culture high performance liquid chromatography.The degradation range of V-5 was detected,and its morphology,biochemical characteristics and molecular phylogenetic analysis of 16S rRNA gene were studied.[Result]V-5 strain could degrade various phenolic acids,the degradation rate of vanillic acid 99.93%,p-hydroxybenzoic acid 99.85%,cinnamic acid 17.44%,Syringate 90.04%,benzoic acid 98.69%,ferulic acid 38.89%respectively;the strain was a short rod shaped Gram-negative bacterium,which could reduce nitrate to nitrite,hydrolyze arginine,assimilate glucose,mannose,gluconate,citric acid,malic acid,citric acid and phenylacetic acid,cytochrome oxidase Positive,very similar to Pseudomonas putida.The phylogenetic analysis of 16S rRNA gene showed that the V-5 strain had the closest relationship with Pseudomonas putida,and the sequence similarity was over 99%.Therefore,the isolated phenolic acid degrading bacteria V-5 was identified as a strain of Pseudomonas putida.[Conclusion]This study provides a theoretical basis for the further development and application of plant allelochemical degrading bacteria and the promotion of sustainable agricultural development.
作者
张春杨
于春雨
高金山
ZHANG Chun-yang;YU Chun-yu;GAO Jin-shan(School of Life Sciences,Shandong University of Technology,Zibo,Shandong 255049)
出处
《安徽农业科学》
CAS
2020年第5期93-95,99,共4页
Journal of Anhui Agricultural Sciences
关键词
花生
连作障碍
酚酸
降解菌
Peanut
Continuous cropping obstacle
Phenolic acids
Degrading bacterium
