摘要
目的对不明原因发热患儿外周血及粪便病原菌分离鉴定,明确感染病因。方法 2018年9月9日,对浙江省嘉兴市第一医院接诊的1例发热患儿血培养及粪便培养的沙门菌进行血清分型和耐药、毒力基因(hilA、ssrA、mgtC、siiD、sopB、spvC)检测及脉冲场凝胶电泳(PFGE)分型。结果患者外周血及粪便中病原菌为印地安纳沙门菌,携带hilA、ssrA、mgtC、siiD、sopB毒力基因,血流和粪便株PFGE聚类相似性为100%。结论该病例是印地安纳沙门菌引起的肠源败血症,应加强本地区基于实验室诊断的沙门菌血清型病例的监测能力。
Objective To identify and analyze the pathogens isolated from peripheral blood and stool samples of a child with fever of unknown origin in Jiaxing, Zhejiang province, and find out source of the infection. Methods Identification,serotyping and pulse field gel electrophoresis(PFGE) were performed for the pathogen strains isolated from peripheral blood and stool samples of the sick child. Polymerase chain reaction(PCR) was performed to detect the virulence genes(hilA, ssrA,mgtC, siiD, sopB, spvC). Results Two pathogen strains isolated from peripheral blood and stool samples of the sick child were identified as Salmonella Indiana. The two strains carried virulence genes hilA, ssrA, mgtC, siiD and sopB, and the band similarity of two strains was 100% indicated by PFGE cluster analysis. Conclusion Fever of this child was caused by the intestinal infection of S. Indiana, further resulting in sepsis. It is necessary to strengthen the laboratory based surveillance for S. Indiana in this area.
作者
高雯洁
王蔚
李萍
王恒辉
郭金磊
高蕾
陈黎霞
Gao Wenjie;Wang Wei;Li Ping;Wang Henghui;Guo Jinlei;Gao Lei;Chen Lixia(Jiaxing Prefecture Center for Disease Control and Prevention,Jiaxing 314050,Zhejiang,China;The First Hospital of Jiaxing,Jiaxing 314000,Zhejiang,China)
出处
《疾病监测》
CAS
2019年第12期1122-1125,共4页
Disease Surveillance
基金
嘉兴市科技计划项目(No.2016AY23089)~~
关键词
印地安纳沙门菌
败血症
鉴定
毒力基因
Salmonella Indiana
Septicemia
Identification
Virulence gene
