摘要
为建立一个稳定、高效的甜椒离体再生体系,本试验以2种基因型的甜椒(野生型CA157和栽培型CA52)为材料,筛选其最佳外植体类型,优化激素配比及浓度来提高愈伤组织的诱导率和子叶节的分化率。通过筛选以野生甜椒CA157、栽培甜椒CA52的子叶节为外植体,结果表明,野生甜椒CA157子叶节愈伤组织诱导率和分化率分别达到82%和73%。栽培甜椒CA52子叶节最高愈伤组织诱导率和分化率分别为81%和70%。诱导愈伤组织最适培养基是MS+2 mg/L TDZ+0.5 mg/L 2,4-D。诱导不定芽形成的最适培养基为MS+2 mg/L TDZ+1.5 mg/L 6-BA,诱导不定芽成活率最高。在不定芽诱导生根培养基(1/2MS+20 g/L蔗糖+1.5 mg/L ABT)上,生根率为67%,每个不定芽生根数最高达到4.2,移栽后再生植株成活率达73%。2个甜椒品种都能得到再生植株而且繁殖系数高。本培养体系适宜甜椒离体再生。通过染色体分析,再生植株染色体数和供体亲本材料一致,初步表明再生植株遗传稳定。
In order to obtain a sweet pepper efficient regeneration system.In this study,two genotypes of sweet pepper(wild type CA157 and cultivated type CA52)were used as materials to select the best explant type and to optimize hormone ratio and concentration to improve callus induction rate and cotyledon node differentiation rate.Cotyledonary segments of wild sweet pepper CA157 and cultivated sweet pepper CA52 were used as explants.The results showed that the induction rate and differentiation rate of callus in the cotyledon section of excited sweet pepper CA157 reached 82%and 73%,respectively.The highest callus inductivity and differentiation rate of the cultivated sweet pepper CA52 were 81%and 70%,respectively.The optimum medium for inducing callus was MS+2 mg/L TDZ+0.5 mg/L 2,4-D.The optimum medium for the induction of adventitious bud formation was MS+2 mg/L TDZ+1.5 mg/L 6-BA,and the highest survival rate of adventitious buds was induced.In the adventitious bud induction rooting medium(1/2MS+20 g/L sucrose+1.5 mg/L ABT),the rooting rate was 67%,the highest number of rooting for each adventitious bud reached 4.2,and the survival rate of the regenerated plants after transplantation was 73%.Both sweet pepper varieties can get regenerated plants and high reproductive coefficient.The culture system is appropriate for ex vivo regeneration of sweet pepper.Chromosomal analysis showed that the number of chromosomes in the regenerated plants was as in the donor parent,indicating that the regenerated plants were genetically stable.
作者
张俊华
苏振华
张泽鑫
李妹芳
郭尚敬
曹雪松
冀芦沙
Zhang Junhua;Su Zhenhua;Zhang Zexin;Li Meifang;Guo Shangjing;Cao Xuesong;Ji Lusha(College of Life Science,Liaocheng University,Liaocheng,252000;College of Agriculture,Liaocheng University,Liaocheng,252000;College of Pharmacy,Liaocheng University,Liaocheng,252000)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第24期8208-8214,共7页
Molecular Plant Breeding
基金
山东省自然科学基金面上项目(ZR2017CM009
ZR2014CM013)资助
