摘要
目的:观察复方苦参注射液对人鼻咽癌CNE-2细胞放射敏感性的影响,并探讨其作用机制。方法:常规体外培养人鼻咽癌CNE-2细胞,采用四甲基偶氮唑盐(MTT)法检测不同浓度复方苦参注射液分别处理CNE-2细胞24、48和72 h后时细胞增殖抑制率,计算复方苦参注射液对CNE-2细胞的半数抑制浓度(IC 50)。CNE-2细胞分为对照组(不照射、不给药)、放射组(给予剂量为2和4 GyX射线照射)和联合组(给予不同浓度复方苦参注射液处理后再接受X射线照射)。MTT法检测各组CNE-2细胞增殖抑制率,流式细胞术检测各组CNE-2细胞凋亡率,克隆形成试验检测各组CNE-2细胞存活率。结果:不同浓度复方苦参注射液处理CNE-2细胞24、48和72 h后,细胞增殖抑制率较未经复方苦参注射液处理的CNE-2细胞升高(P<0.05),且呈剂量依赖性;复方苦参注射液处理48 h时细胞增殖抑制率高于24 h时(P<0.05)。放射组CNE-2细胞凋亡率高于对照组(P<0.05),联合组CNE-2细胞凋亡率较放射组升高(P<0.05);联合组CNE-2细胞存活率低于放射组(P<0.05)。结论:复方苦参注射液能促进放射线诱导的鼻咽癌CNE-2细胞凋亡,从而对CNE-2细胞具有放射增敏作用。
Objective:To observe the effect of Compound Sophora Flavescens Injection on the radiosensitivity of the human nasopharyngeal carcinoma CNE-2 cells,and to explore its mechanism.Methods:The human nasopharyngeal carcinoma CNE-2 cells were cultured in vitro.The inhibitory rates ofProliferation of CNE-2 cells after treated with different concentrations of Compound Sophora Flavescens Injection for 24,48,and 72 h were detected by MTT method.The semi-inhibitory concentration(IC 50)of Compound Sophora Flavescens Injection on CNE-2 cells was calculated.The CNE-2 cells were divided into control group(without irradiation and adminnistration),irradiation group(treated with 2 and 4 Gy X-ray irradiation)and combination group(treated with different concentrations of Compound Sophora Flavescens Injection and then received X-ray irradiation).MTT assay was used to detect the inhibitory rates ofProliferation of the CNE-2 cells in various groups,flow cytometry was used to detect the apoptotic rates of the CNE-2 cells in various groups,and clone formation test was used to detect the survival rates of the CNE-2 cells in various groups.Results:After treated with different concentrations of Compound Kushen Injection for 24,48 and 72 h,the inhibitory rates ofProliferation of the CNE-2 cells were increased compared with the CNE-2 cells without Compound Kushen Injection treatment(P<0.05)in an dose-dependent manner;the inhibitory rate ofProliferation of the CNE-2 cells after treated with Compound Kushen injection for 48 h was higher than 24 h(P<0.05).The apoptotic rate of the CNE-2 cells in radiation group was higher than that in control group(P<0.05);the apoptotic rate of the CNE-2 cells in combination group was higher than that in radiation group(P<0.05);the survival rate of the CNE-2 cells in combination group was lower than that in radiation group(P<0.05).Conclusion:Compound Sophora Flavescens Injection canPromote the irradiation-induced apoptosis of nasopharyngeal carcinoma CNE-2 cells,therefore,Compound Sophora Flavescens Injection has the radiosensitization effect on the CNE-2 cells.
作者
郑剑霄
詹文婷
周晓春
孔怡琳
高蕾
李工
王苏美
ZHENG Jianxiao;ZHAN Wenting;ZHOU Xiaochun;KONG Yilin;GAO Lei;LI Gong;WANG Sumei(Department of Radiotherapy,Sencond Affiliated Hospital,Guangzhou University of Traditional Chinese Medicine,GuangdongProvincial Hospital of Traditional Chinese Medicine,Guangzhou 510120,China;Department of Oncology,Sencond Affiliated Hospital,Guangzhou University of Traditional Chinese Medicine,GuangdongProvincial Hospital of Traditional Chinese Medicine,Guangzhou 510120,China)
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2019年第6期1384-1388,I0020,共6页
Journal of Jilin University：Medicine Edition
基金
广东省卫计委医学科研基金资助课题(A2018251)
关键词
复方苦参注射液
细胞凋亡
放射增敏
鼻咽肿瘤
Compound Sophora Flavescens Injection
apoptosis
radiosensitization
nasopharyngeal neoplasms
