摘要
目的 明确尿酸氧化酶(urate oxidase)基因(Uox)敲除(Uox-/-)自发高尿酸血症对C57BL/6 J小鼠体重、血压及血液生理生化等基础生物学指标的影响。方法 选取6、12周龄C57BL/6 J野生型(Uox+/+)、尿酸氧化酶基因杂合缺失(Uox+/-)和尿酸氧化酶基因敲除(Uox-/-)雌性和雄性小鼠,测量体重、血压,并检测主要血生理、生化指标。结果 体重结果显示,雄性和6周龄雌性Uox-/-小鼠体重显著低于Uox+/+对照小鼠(P<0. 05);雄性Uox+/+和Uox+/-小鼠体重显著高于同周龄雌性小鼠(P<0. 05),而不同性别Uox-/-小鼠体重之间无统计学差异。血压结果显示,6周龄雌性Uox-/-小鼠和12周龄雄性Uox-/-小鼠血压显著高于同周龄Uox+/+和Uox+/-小鼠(P<0. 05)。血液学生理指标结果显示,Uox-/-小鼠红细胞计数(RBC)、红细胞压积(HCT)、平均红细胞体积(MCV)和血红蛋白(HGB)水平显著低于Uox+/+小鼠(P<0. 05),平均血红蛋白浓度(MCHC)和血小板压积(PCT)高于Uox+/+小鼠(P<0. 05);Uox+/-小鼠RBC、HCT和HGB水平也低于Uox+/+小鼠(P<0. 05),PCT高于Uox+/+小鼠(P<0. 05)。血液学生化指标结果显示,Uox-/-小鼠白蛋白(ALB)、总胆红素(TBIL)和直接胆红素(DBIL)水平明显低于Uox+/+小鼠(P<0. 05),尿酸(UA)、肌酐(CREA)、尿素氮(BUN)、总胆固醇(CHOL)和低密度脂蛋白(LDL)水平均显著高于Uox+/+小鼠(P<0. 05)。结论 建立了Uox基因敲除C57BL/6 J小鼠基础生物学数据,Uox-/-自发高尿酸血症能够引起小鼠体重、血压、肾功能及脂类代谢等变化。
Objective To determine the effects of urate oxidase(Uox)gene knockout(Uox-/-)-induced spontaneous hyperuricemia on the body weight,blood pressure,and blood physiological and biochemical parameters of C57BL/6 J mice.Methods Both female and male C57BL/6 J mice at six-and twelve-week-old were contained,including wild-type(Uox+/+),urate oxidase knockout heterozygotes(Uox+/-)and urate oxidase knockout homozygous(Uox-/-)mice.Mice body weight,blood pressure,and major blood physiological and biochemical parameters were observed.Results The average body weight of the 6-week-old female Uox-/-mice and all male Uox-/-mice was significantly lower than that of the Uox+/+mice(P<0.05).The body weight of the male Uox+/+and Uox+/-mice was significantly higher than that of the female mice of the same genotype and age(P<0.05),but the body weight showed no statistical differences between the Uox-/-mice of different sex.Compared with Uox+/+and Uox+/-mice of the same age,6-week-old female Uox-/-mice and 12-week-old male Uox-/-mice had significantly higher blood pressure(P<0.05).Blood physiological parameters showed that the red blood cell(RBC),hematocrit(HCT),mean corpuscular volume(MCV),and hemoglobin(HGB)of the Uox-/-mice were significantly lower than those of the Uox+/+mice(P<0.05),and their mean corpuscular hemoglobin concentration(MCHC)and plateletcrit(PCT)were higher than those of the Uox+/+mice(P<0.05).Compared with the Uox+/+mice,RBC,HCT and HGB of the Uox+/-mice were significantly decreased(P<0.05),while PCT was significantly increased(P<0.05).The blood biochemical parameters showed that albumin(ALB),total bilirubin(TBIL),and direct bilirubin(DBIL)of the Uox-/-mice were lower than those of the Uox+/+mice(P<0.05),and their uric acid(UA),creatinine(CREA),blood urea nitrogen(BUN),cholesterol(CHOL),and low density lipoprotein(LDL)were significantly higher than those of the Uox+/+mice(P<0.05).Conclusions The basic biological data of C57BL/6 J mice with the urate oxidase gene knockout were established.Uox-/--induced spontaneous hyperuricemia could cause abnormal changes in body weight,blood pressure,kidney function,and lipid metabolism of mice.
作者
潘明子
贺玉伟
李海龙
梁楠
李长贵
PAN Mingzi;HE Yuwei;LI Hailong;LIANG Nan;LI Changgui(The Affiliated Hospital of Qingdao University,Qingdao 266021,China;Institute of Metabolic Diseases,Qingdao University,Qingdao 266021)
出处
《中国比较医学杂志》
CAS
北大核心
2019年第11期71-77,共7页
Chinese Journal of Comparative Medicine
基金
山东省自然科学基金(ZR2018ZC1053)
山东省重大科技创新工程项目(2018CXGC1207)
中国博士后科学基金面上项目(2018M630755)
关键词
尿酸氧化酶
基因敲除
高尿酸血症
血压
血生理生化指标
小鼠
urate oxidase
gene knockout
hyperuricemia
blood pressure
blood physiological and biochemical parameters
mouse
