摘要
本实验研究了昆仑雪菊总黄酮(Kunlun chrysanthemum total flavonoids,KCTF)的抗氧化活性、抑菌性、对四氯化碳(CCl4)诱导的小鼠肝损伤的保护作用,及对肿瘤细胞增殖的抑制能力。通过分析KCTF的自由基清除能力及对体内超氧化物歧化酶活力和丙二醛含量的影响评价其抗氧化能力;采用CCl4诱导建立小鼠急性肝损伤模型检测KCTF的解毒作用;选取HeLa细胞和Eca-109食管癌细胞观察KCTF对肿瘤细胞增殖的抑制能力。结果表明:KCTF具有显著的抗氧化活性;KCTF能够抑制革兰氏阳性和革兰氏阴性菌的生长;然而KCTF对CCl4诱导的小鼠急性肝损伤没有明显的保护作用;KCTF能够抑制HeLa细胞和Eca-109细胞的增殖,半抑制质量浓度平均值分别为(133.60±0.12)、(192.00±0.08)μg/mL。结论:KCTF由于其天然的抗氧化、抑菌和抑制肿瘤细胞增殖作用,在功能性食品工业中具有潜在的应用价值。
In this study, the antioxidant and antibacterial properties of total flavonoids from Kunlun chrysanthemum flower(KCTF) were evaluated together with its protective effect on liver injury induced by carbon tetrachloride in mice and anti-proliferative effect on tumor cells. The antioxidant capacity was measured by free radical scavenging capacity in vitro, as well as superoxide dismutase activity and malondialdehyde content in vivo. Moreover, the anti-proliferative activity was determined using HeLa cells and esophageal cancer Eca-109 cells. Results indicated that KCTF had significant antioxidant activity and inhibited the growth of both Gram-positive and Gram-negative bacteria. However, KCTF had no significant hepatoprotective effect. KCTF possessed potent inhibitory activity with average half-inhibitory concentration(IC50) values of(133.60 ± 0.12) and(192.00 ± 0.08) μg/mL for HeLa and Eca-109 cells, respectively. Therefore, KCTF may have potential application in functional food industries due to its antioxidant, bacteriostatic and anti-proliferative effects.
作者
敬思群
翟红月
苏乐萍
任志艳
王银娜
晏良军
JING Siqun;ZHAI Hongyue;SU Leping;REN Zhiyan;WANG Yinna;YAN Liangjun(Yingdong College of Food Science and Engineering,Shaoguan University,Shaoguan 512005,China;College of Life Sciences and Technology,Xinjiang University,ürümqi 830046,China;Department of Pharmaceutical Sciences,UNT System College of Pharmacy,University of North Texas Health Science Center,Fort Worth 76107,USA)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第21期177-187,共11页
Food Science
基金
2015年自治区研究生科研创新项目(XJGRI2015024)
关键词
昆仑雪菊总黄酮
抗氧化活性
抑菌性
抑制增殖能力
肝损伤保护作用
total flavonoids from Kunlun chrysanthemum
antioxidant activity
antibacterial activity
anti-proliferative effect
protective effect on liver injury
