摘要
目的研究前列腺素E2(PGE2)对小鼠骨髓来源树突状细胞(BMDC)的表型及趋化功能的影响。方法分离并培养小鼠BMDC,分为(0、 1、 5)μg/mL PGE2组、(0、 1、 5)μg/mL PGE2联合1μg/mLLPS组。流式细胞术检测BMDC表面分子CD40、 CD86、主要组织相容性复合体Ⅱ(MHCⅡ)及CC趋化因子受体7(CCR7)的表达, Western blot法检测BMDC的CCR7蛋白水平, TranswellTM检测BMDC的迁移能力, CCK-8法检测BMDC存活率的变化。结果 1μg/mL PGE2上调BMDC表面分子及CCR7表达,并促进BMDC迁移, 5μg/mL PGE2下调BMDC表面分子及CCR7表达并抑制BMDC迁移;PGE2剂量变化并不影响BMDC的存活率。结论低剂量PGE2促进BMDC的迁移,高剂量PGE2抑制BMDC的迁移, PGE2对BMDC的迁移有双重调节作用。
Objective To explore the effect of prostaglandin E2(PGE2) on the phenotype and chemotaxis of mouse bone marrow-derived dendritic cells(BMDCs). Methods BMDCs isolated from murine bone marrow and cultured in vitro were divided into 6 groups(0 μg/mL PGE2 group, 1 μg/mL PGE2 group, 5 μg/mL PGE2 group, 0 μg/mL PGE2 plus LPS group, 1 μg/mL PGE2 plus LPS group, 5 μg/mL PGE2 plus LPS group). The expression of surface makers CD40, CD86, major histocompatibility complex Ⅱ(MHCⅡ), CC chemokine receptor 7(CCR7) were detected by flow cytometry. The expression of CCR7 protein was detected by Western blot analysis. The migration ability of BMDCs was detected by TranswellTM assay. The survival rate of BMDCs was detected by CCK-8 assay. Results PGE2 of 1 μg/mL increased the expression of surface molecules on BMDCs and promoted the migration ability of BMDCs. PGE2 of 5 μg/mL reduced the expression of surface molecules on BMDCs and inhibited the migration ability of BMDCs. The change of PGE2 concentration did not affect the survival rate of BMDCs. Conclusion PGE2 was demonstrated a dual regulatory effect on the migration of BMDCs. Low concentration of PGE2 can promote the migration ability of BMDCs, while high concentration of PGE2 shows contrary effect.
作者
黄洁
刁戈
韩健
郭建新
HUANG Jie;DIAO Ge;HAN Jian;GUO Jianxin(Daping Hospital of Army Medical University,Chongqing,400000,China)
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2019年第7期583-588,共6页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金(81272864)
重庆市自然科学基金重点项目(CSTC2011BA5008)
