摘要
目的:研究苍术-玄参药对有效部位配伍后对小鼠糖尿病的治疗作用,探讨其可能的降糖作用机制。方法:将苍术-玄参药对2个主要有效部位(多糖部位、正丁醇部位)按配比分为:1∶1、1∶2、2∶1,共3组。采用腹腔注射链脲佐菌素(STZ)联合高脂饲料建立小鼠糖尿病模型,以空腹血糖(FBG)为考察指标,考察有效部位最优配比。通过测定肝脏组织中的丙二醛(MDA)水平以及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活性,探讨可能的降糖机制。结果:经多元统计分析,多糖-正丁醇部位2∶1配伍时,对糖尿病小鼠降糖效果最佳。多糖和正丁醇部位配伍可以显著降低MDA水平,明显增强GSH-Px和SOD活性。结论:苍术-玄参药对中多糖和正丁醇部位按照2∶1比例配伍降糖作用最为显著,其降糖机制可能与提高小鼠机体抗氧化能力有一定的相关性。
Objective:To study the therapeutic effect of effective parts of Rhizoma Atractylodis-Radix Scrophulariae on diabetic mice and explore its possible hypoglycemic mechanism.Methods:Two active parts of Rhizoma Atractylodis-Radix Scrophulariae(polysaccharide and n-butanol parts) were divided into 3 groups according to their ratio:1∶1,1∶2,2∶1.The diabetic mice model has been established by intraperitoneal injection of streptozotocin(STZ) combined with high-fat and high-sugar diet.Taking fasting blood glucose(FBG) as an index,the optimal proportion of effective fractions was investigated.The levels of malondialdehyde(MDA) and the activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) in liver tissue were measured to explore the possible hypoglycemic mechanism.Results:Multivariate statistical analysis showed that the best hypoglycemic effect was obtained in diabetic mice when the polysaccharide-n-butanol fraction was 2∶1.The compatibility of polysaccharides and n-butanol fractions could significantly reduce the level of MDA and increase the activity of GSH-Px and SOD.Conclusion:The most significant hypoglycemic effect of polysaccharides and n-butanol fractions in 2∶1 ratio was observed.Its hypoglycemic mechanism may be related to the improvement of antioxidant capacity in mice.
作者
杨惠
刘颖新
孔兴欣
陈容
蒋梅香
刘利利
Yang Hui;Liu Yingxin;Kong Xingxin;Chen Rong;Jiang Meixiang;Liu Lili(Hunan Traditional Chinese Medical College,Zhuzhou 412012,China)
出处
《亚太传统医药》
2019年第10期20-22,共3页
Asia-Pacific Traditional Medicine
基金
湖南省教育厅科学研究项目(优秀青年项目)(15B182)
湖南省教育厅科学研究项目(17C1253)
关键词
苍术-玄参药对
有效部位
配伍
降糖
机制
Drug Couple of Rhizoma Atractylodis-Radix Scrophulariae
Active Part
Compatibility
Hypoglycemic
Mechanism
