摘要
优化根瘤菌(Rhizobium NG10)产多糖的培养条件,提高胞外多糖(extracelluar polysaccharides of Rhizobium,REPS)产量,并探究其抗肿瘤活性。通过单因素实验、Plackett-Burman实验和Box-Behnken实验等对根瘤菌产胞外多糖发酵培养基配方进行优化,并采用MTT法检测多糖对人肝癌细胞的抑制作用。最终优化得到的最优培养基配方为:麦芽糖22.5 g/L,大豆蛋白胨9.5 g/L,MgSO 4·7H 2O 0.2 g/L,KH 2PO 4 0.41 g/L,NaCl 0.1 g/L,此时胞外多糖产量为5.05 g/L,相比原始提高了45.95%;REPS对肝癌细胞Hep G2的抑制呈一定的量效关系,培养时间为48 h时IC 50为393.3μg/mL,肝癌细胞抑制率达到56.74%。
In this study,the optimal the culture conditions for production of extracellular polysaccharides by Rhizobium sp.NG10 were determined.Besides,the inhibitory effect of the polysaccharides on human hepatocellular carcinoma cells was studied by MTT assay.The optimal medium composition for extracellular polysaccharides production were 22.5 g/L maltose,9.5 g/L soybean peptone,0.2 g/L MgSO 4·7H 2O,0.41 g/L KH 2PO 4 and 0.1 g/L NaCl.Under these conditions,the yield of the polysaccharides was increased 45.95%with titer of 5.05 g/L.Moreover,the inhibitory effect of the polysaccharides on human hepatocellular carcinoma cells exhibited a dose-effect relationship.When the human hepatocellular carcinoma cells were cultured with the polysaccharides for 48 h,the IC 50 was 393.3μg/mL,and the inhibition rate was 56.74%.
作者
贾玉香
耿晓琦
黄正梅
杜鹏
郑宇
宋佳
JIA Yuxiang;GENG Xiaoqi;HUANG Zhengmei;DU Peng;ZHENG Yu;SONG Jia(Key Laboratory of Industrial Fermentation Microbiology,Ministry of Education,College of Biotechnology,Tianjin University of Science and Technology,Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control,Tianjin 300457,China;Onlystar Biotechnology Company Ltd,Dezhou 253000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2019年第20期144-148,共5页
Food and Fermentation Industries
基金
天津科技大学青年创新基金项目(2016LG10)
天津市高等学校大学生创新创业训练计划项目(201810057079)
天津市食品绿色制造及安全校企协同创新实验室建设(17PTSYJC0080)
工业微生物优良菌种选育与发酵技术公共服务平台项目(17PTGCCX00190)
关键词
根瘤菌
胞外多糖
培养基优化
体外实验
抗肿瘤活性
Rhizobium sp.NG10
extracellular polysaccharide
medium optimization
in vitro experiment
anti-tumor activity
