摘要
目的采用离子色谱法优化测定小儿复方氨基酸注射液中的氨含量。方法采用阳离子交换色谱柱RFICTM IonPac^■CG16(4 mm×50 mm),保护柱CS16(4 mm×250 mm);以20 mmol·L^-1甲基磺酸为流动相A,以100 mmol·L^-1甲基磺酸为流动相B,梯度洗脱,流速为1.0 mL·min^-1;检测器为电导检测器(带抑制器CERS 300,4 mm);进样体积25μL。结果氨的线性范围为0.149 9~5.997 9μg·mL^-1(r=0.998 4),平均回收率为96.8%,RSD为2.82%。结论该法专属性高、结果准确可靠,可用于小儿复方氨基酸注射液中氨的含量控制。
OBJECTIVE To use ion chromatography method to optimize the assay of ammonium in Pediatric Compound Aminoacid injection.METHODS The IC method was performed on a cation exchange column RFICTM IonPac^■CG16(4 mm×50 mm),CS16(4 mm×250 mm)by gradient elution at a flow rate of 1.0 mL·min^-1.The mobile phase A was 20 mmol·L^-1 methanesulfonic acid,the mobile phase B was 100 mmol·L^-1 methanesulfonic acid.The detector was conductivity detector with a suppressor(CERS 300,4 mm)and the injection volume was 25μL.RESULTS The linear range of the ammonium was 0.149 9-5.997 9μg·mL^-1(r=0.998 4).The average recovery of it was 96.8%and the RSD was 2.82%.CONCLUSION The method was specific,accurate and suitable for the assay of ammonium for quantitative control of Pediatric Compound Aminoacid injection.
作者
傅萍
刘峰
谢华
FU Ping;LIU Feng;XIE Hua(Sichuan Food and Drug Inspection and Testing Institute,Chengdu 611731,China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2019年第18期2295-2298,共4页
Chinese Journal of Modern Applied Pharmacy
关键词
离子色谱法
阳离子交换柱
电导检测器
小儿复方氨基酸注射液
氨
含量测定
ion chromatography
cation exchange column
conductivity detector
Pediatric Compound Aminoacid injection
ammonium
content determination
