摘要
目的研究氯喹对戊四氮(PTZ)致痫大鼠脑内星形胶质细胞介导的腺苷激酶(ADK)及谷氨酸(Glu)表达的影响,探讨氯喹在控制癫痫发作中的治疗作用及机制。方法30只健康雄性SD大鼠随机分为对照组、戊四氮(PTZ)致痫组和氯喹干预组,每组10只。观察大鼠行为表现,记录脑电改变,免疫组化法检测3组大鼠脑内ADK和Glu的表达水平。结果对照组大鼠无痫样发作,戊四氮(PTZ)致痫组大鼠癫痫发作严重(Ⅳ~Ⅴ级),脑电图记录呈频发高幅的癫痫样波,氯喹干预组大鼠的痫样发作有所减轻(Ⅰ~Ⅲ级),脑电图显示癫痫样波幅低且缓。戊四氮(PTZ)致痫组大鼠脑内ADK和Glu表达增强,与对照组比较差异有统计学意义(P<0.01),采用氯喹干预后,大鼠脑内ADK及Glu表达降低,与癫痫组相比差异均有统计学意义(P<0.05)。结论氯喹可减轻癫痫大鼠的痫样发作,抑制脑内ADK的表达水平,并能降低Glu的含量。
Objective To observe the effects of chloroquine on adenosine kinase(ADK)and Glutamate(Glu)in cerebral cortex and hippocampal of rats with epilepsy induced by pentylenetetrazole,to explore the role of chloroquine in epileptogenesis.Methods Thirty male adult Sprague-Dawley(SD)rats were randomly divided into control group(n=10),pentylenetetrazole(PTZ)group(n=10)and chloroquine intervening group(n=10).The behavior and electroencephalogram of rats were observed and recorded.The expression of ADK and Glu in cerebral cortex and hippocampus was examined with immunohistochemistry.Results The control group showed no seizure activity.Severe seizure activity was observed in PTZ group(Ⅳ-Ⅴdegree),while slight seizure activity(Ⅰ-Ⅲdegree)appeared in the chloroquine intervening group.Electroencephalogram studies showed high amplitudes with fast frequencies in the PTZ group,low amplitudes and slow frequencies in the chloroquine intervening group.The expressions of ADK and Glu in the PTZ group were stronger than that of the control group(P<0.01),and significant differences were observed between the chloroquine intervening group and the PTZ group(P<0.05).Conclusion Chloroquine could alleviate the seizure activities induced by PTZ in rats,inhibit the expression level of ADK and Glu.
作者
于宁
赵玉红
荆丽丽
吴淑华
曹璋
YU Ning;ZHAO Yuhong;JING Lili;WU Shuhua;CAO Zhang(Department of Pathology,Binzhou Medical University Hospital,Binzhou 256603,P.R.China;Department of Pathology,Yantai Affiliated Hospital of Binzhou Medical University;Department of Pathology,Binzhou Medical University)
出处
《滨州医学院学报》
2019年第4期243-246,共4页
Journal of Binzhou Medical University
基金
山东省医药卫生科技计划(2016WS0014)
