摘要
目的:比较荧光染色法与KOH湿片法在皮肤浅表真菌感染中的应用价值。方法:收集我科门诊疑似皮肤浅表真菌感染患者192例,其中体股部98例,手足部52例,指(趾)甲42例,标本均分为2份,分别行荧光染色法与KOH湿片法,比较其检测结果。结果:荧光染色法阳性141例,阴性51例,阳性率为73.4%;其中体股部阳性77例,阴性21例,阳性率为78.6%;手足部阳性39例,阴性13例,阳性率为75.0%;指(趾)甲部阳性25例,阴性17例,阳性率为59.5%。KOH湿片法阳性103例,阴性89例,阳性率为53.6%;其中体股部阳性59例,阴性39例,阳性率为60.2%;手足部阳性28例,阴性24例,阳性率为53.8%;指(趾)甲部阳性16例,阴性26例,阳性率为38.1%。荧光染色法真菌检测阳性率明显高于KOH湿片法(2=16.23,P<0.05)。荧光染色法检测体股部、手足部及指(趾)甲部相对KOH湿片法的特异度分别为53.8%、54.2%、65.4%,灵敏度均为100%。结论:荧光染色法对初学者操作简单、准确性高、患者可分辨真菌形态,治疗依从性高,适合临床应用。
Objective:To compare the application value of the fluorescence staining method with KOH wet method in superficial fungal infections of the skin. Methods: One hundred and ninety two outpatient suspected patients of superficial fungal skin infection were collected. Specimens were divided into two parts, and detected by fluorescent staining and KOH wet method at the same time, then the test results were compared. Result: With fluorescent staining method: the positive rate was 73.4%(141/192). In the patients involved with trunk and femoral-genital region, the positive rate was 78.6%(77/98). Whereas in patients involved with hands and feet, positive rate was 75.0%(39/52). As regards to the fingers/toes region, positive rate was 59.5%(25/42). With KOH wet positive method:the positive rate was 53.6%(103/192). The positive rate in the trunk region,in hands and feet and in fingers/toes was 60.2%(59/98),53.8%(28/52),38.1%(16/42), respectively. The positive rate of fluorescent fungus staining test was significantly higher than those of KOH wet method(χ^2=16.23,P<0.05). Relative to KOH wet method,the specificity of fluorescent staining method to detect regions of trunk, feet and fingers/toes region were 53.8%, 54.2% and 65.4%,respectively, and the sensitivity was 100%. Conclusion:As for in-experienced technician, fluorescent staining is easy to perform, with a high accuracy. The fungi figures are easily distinguishable even to patients, resulting in high patient compliance. Therefore, it is applicable to clinic work.
作者
唐宏伟
周敏
TANG Hong-wei;ZHOU Min(Dermatology Department,Yongzhou Third People’s Hospital,Yongzhou 425100,China)
出处
《皮肤性病诊疗学杂志》
2019年第4期225-228,共4页
Journal of Diagnosis and Therapy on Dermato-venereology
基金
广东省医学科学技术研究基金(编号:A2016239、A2016269)
关键词
荧光染色法
KOH湿片法
真菌感染
fluorescent staining
KOH wet film method
mycotic infection
