摘要
目的建立HPLC测定香砂养胃丸(水丸)特征图谱的方法,提供可靠的的质量控制方法。方法Wondasil C18-WR(4.6 mm×250 mm,5μm)色谱柱,甲醇-水为流动相,梯度洗脱(0~25 min,20%A→65%A;25~45 min,65%A→76%A;45~60 min,76%A),流速1.0 ml/min,测定波长254 nm,柱温30℃。结果18批香砂养胃丸特征图谱标定了共有峰12个,指认了橙皮苷(峰4)、和厚朴酚(峰8)、厚朴酚(峰11)、α-香附酮(峰12)4个特征峰,方法精密度、稳定性、重复性、耐用性考察结果均满意。结论该方法简便、准确、可靠,可用于香砂养胃丸的质量控制。
Objective To establish an HPLC method for the determination of characteristic chromatogram of Xiangsha Yangwei Pills(water pills).Methods The analysis was carried out on an analytical column of Wondasil C18-WR(4.6 mm×250 mm,5μm),using methanol(A)-water(B)at a flow rate of 1.0 ml/min as mobile phase(gradient elution,0-25 min,20%A→65%A;25-45 min,65%A→76%A;45-60 min,76%A).The detection wavelength was 254 nm and the column temperature was 30℃.Results Twelve common peaks were found in characteristic chromatograms of 18 batches of Xiangsha Yangwei Pills,and four of them were identified as hesperidin(peak 4),honokiol(peak 8),magnolol(peak 11),α-cyperone(peak 12).The precision,stability,repeatability and durability of the method were all satisfactory.Conclusion The proposed method is simple,accurate and reliable,and can be used for the quality control of Xiangsha Yangwei Pills.
作者
程雅婷
王晓云
王学涛
王艳博
刘凯娜
张上上
杨新宇
郑斓
CHENG Ya-ting;WANG Xiao-yun;WANG Xue-tao;WANG Yan-bo;LIU Kai-na;ZHANG Shang-shang;YANGXin-yu;ZHENG Lan(Qinhuangdao Institute for Food and Drug Control,Qinhuangdao 066000,China;Chiatai Qingchunbao Pharmaceutical Co.,Ltd.,Hangzhou 310000,China)
出处
《食品与药品》
CAS
2019年第4期273-278,共6页
Food and Drug
关键词
特征图谱
香砂养胃丸
橙皮苷
厚朴酚
和厚朴酚
Α-香附酮
characteristic chromatogram
Xiangsha Yangwei Pills
hesperidin
magnolol
honokiol
α-cyperone
