摘要
目的测定溃疡灵胶囊中人参皂苷Rg1 的含量,控制药品质量,增强标准的约束力.方法采用高效液相色谱法(HPLC法)测定溃疡灵胶囊中人参皂苷Rg1 的含量,色谱条件:色谱柱:Hypersil BDS C18(250 mm×4.6 mm,5 μm);流动相:乙腈-0.05%磷酸溶液(21:79),检测波长:203 nm;柱温:30℃;流速:1.0 ml/min;理论塔板数按人参皂苷Rg1 计算不得低于5000.结果人参皂苷Rg1 在0.852~4.260μg范围内线性关系良好,r=0.9999.人参皂苷Rg1 平均回收率为97.86%,RSD=1.65%.结论 HPLC法稳定可靠,方法准确,重复性好,可用于溃疡灵胶囊的质量控制.
Objective To detect the content of ginsenoside Rg1 in Kuiyangling capsule to control the quality of the product and enhance the binding force of the standard.Methods The content of ginsenoside Rg1 in Kuiyangling capsule was determined by HPLC.Chromatographic conditions were as follows:chromatographic column Hypersil BDS C18(250 mm×4.6 mm,5μm);mobile phase acetonitrile-0.05%phosphoric acid solution(21 :79),detection wave﹣length:203 nm,column temperature 30℃,flow rate 1.0 ml/min.The theoretical plate number calculated by ginsen﹣oside Rg1 could not be less than 5000.Results Ginsenoside Rg1 had a good linear relationship within the rage 0.852~4.260 μg(r=0.9999).The average recovery rate of ginsenoside Rg1 was 97.86%,RSD=1.65%.Conclusion HPLC method is stable,reliable,accurate and reproducible,which can be used for the quality control of Kuiyangling capsule.
作者
潘彦荣
刘艳霞
常晓平
Pan Yanrong;Liu Yanxia;Chang Xiaoping(Xuchang Food and Drug Inspection and Testing Center,Xuchang 461000, China)
出处
《医学新知》
CAS
2019年第4期411-413,F0003,共4页
New Medicine
