摘要
目的 探究糖皮质激素诱导蛋白激酶1(serum and glucocorticoid-inducible kinase 1, SGK1)基因沉默对人前列腺癌细胞生长抑制的作用。方法 选取人前列腺癌PC-3细胞,将RNAi重组质粒(PSGK1-RNAi)通过脂质体转染至PC-3细胞,再用G418筛选稳定转染的SGK1基因沉默的PC-3细胞作为SGK1基因沉默组,并将正常培育的PC-3细胞和空质粒稳定转染的PC-3细胞分别作为阴性对照组与阳性对照组。检测培育48 h、72 h、96 h的细胞增殖能力,计算培育48 h的不同细胞周期表达水平和细胞凋亡情况。结果 培育48 h、72 h及96 h,三组PC-3细胞水平差异有统计学意义( P <0.05);与SGK1基因沉默组比较,培育48 h、72 h及96 h的阳性对照组、阴性对照组PC-3细胞水平升高,差异具有统计学意义( P <0.05)。三组G1期和S期细胞所占比例比较差异有统计学意义( P <0.01);与SGK1基因沉默组比较,阳性对照组、阴性对照组G1期细胞所占比例下降,S期细胞所占比例升高,差异具有统计学意义( P <0.01)。三组PC-3细胞凋亡率比较差异有统计学意义( P <0.01);与SGK1基因沉默组比较,阳性对照组、阴性对照组细胞凋亡率下降,差异有统计学意义( P <0.001)。结论 SGK1基因沉默能抑制PC-3细胞增殖,诱发PC-3细胞凋亡,可作为基因治疗前列腺癌的有效靶点。
Objective To explore inhibitory effect of serum and glucocorticoid-inducible kinase 1 (SGK1) gene silencing on growth of human prostate cancer cells. Methods The PC-3 human prostate cancer cells were selected. RNAi recombinant plasmid (PSGK1-RNAi) was transfected into PC-3 cells by liposome, and then stably transfected SGK1 gene silencing PC-3 cells (SGK1 gene silencing group) were screened by G418. Normally-cultured PC-3 cells and empty plasmid stably transfected PC-3 cells were enrolled as negative control group and positive control group. The cell proliferation ability at 48h, 72h and 96h after culture was detected. The expression level at different stages of cell cycles and apoptosis at 48h after culture were calculated. Results At 48h, 72h and 96h after culture, there were significant differences in PC-3 cells levels among the three groups ( P <0.05). Compared with SGK1 gene silencing group, levels of PC-3 cells in positive control group and negative control group were increased at 48h, 72h and 96h after culture, suggesting significant differences ( P <0.05). There were significant differences in proportion of cells in G1 phase and S phase among three groups ( P <0.01). Compared with SGK1 gene silencing group, proportion of cells in G1 phase of positive control group and negative control group was decreased, while proportion of cells in S phase was increased, suggesting significant differences ( P <0.01). There were significant differences in apoptosis rate of PC-3 cells among three groups ( P <0.01). Compared with SGK1 gene silencing group, apoptotic rate of positive control group and negative control group was decreased, and there were significant differences ( P <0.001). Conclusion SGK1 gene silencing can inhibit proliferation of PC-3 cells and induce apoptosis of PC-3 cells. Therefore, it can be used as an effective target for gene therapy of prostate cancer.
作者
彭艳
高雷
田春琴
刘晓明
崔立春
PENG Yan;GAO Lei;TIAN Chun-qin;LIU Xiao-ming;CUI Li-chun(Department of Oncology, Chang'an Hospital, Xi'an 710000, China;Department of Endocrinology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710000, China;Department of Emergency, Traditional Chinese Medicine Hospital of Lin'an District, Hangzhou 311300, China)
出处
《临床误诊误治》
2019年第9期95-98,共4页
Clinical Misdiagnosis & Mistherapy
基金
陕西省社会发展科技攻关项目(2015SF005)
