摘要
【目的】解淀粉芽胞杆菌菌株FJAT-8754是一株高产纤维素酶、淀粉酶的芽胞杆菌,具有在复合饲料酶制剂生产中的应用潜力。为深入了解该菌株的发酵进程,以便实现对发酵过程进行最优化控制。【方法】采用50L发酵罐发酵菌株FJAT-8754,分析发酵过程中菌体数量、pH值、底物浓度、纤维素酶和淀粉酶等的动态变化,并选用Logistic方程和Luedeking-Piret方程构建发酵动力学模型。【结果】根据菌株FJAT-8754发酵过程中各参数变化动态建立了模拟菌体生长、纤维素酶与淀粉酶产生和底物消耗的动力学模型,并应用SPSS 16.0软件对数据进行计算与分析,Origin 9.0软件经非线性拟合与优化,获得了最佳模型参数值。【结论】所构建的动力学模型的计算值与实验值拟合良好,较好地反映了菌株FJAT-8754发酵过程中菌体生长、底物消耗和酶合成的动力学特征。菌株FJAT-8754发酵过程中产物的合成属于生长部分偶联型。
【Objective】To construct a kinetic model for the optimized fermentation of Bacillus amyloliquefaciens FJAT-8754,which was capable of effectively producing cellulase and amylase.【Method】A 50 L fermenter experiment was conducted to study the cellular growth,pH value,enzyme production and carbohydrate consumption of the fermentation and for subsequent process optimization.A kinetic model was established using the logistic and Luedeking-Piret equations,and experimental data calculated and analyzed with SPSS 16.0 software.【Result】 The optimal fermentation conditions fitted well in the non-linear model as shown by Origin 9.0 software.【Conclusion】The kinetic model obtained accurately reflected the performance of the fermentation.The cellulase and amylase were products of FJAT-8754 metabolism in the culture.
作者
葛慈斌
蓝江林
刘波
王凯
郑雪芳
GE Ci-bin;LAN Jiang-lin;LIU Bo;WANG Kai;ZHENG Xue-fang(Institute of Agricultural Bio-resources,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2019年第6期697-704,共8页
Fujian Journal of Agricultural Sciences
基金
福建省自然科学基金项目(2017J01048)
福建省财政专项——福建省农业科学院科技创新团队建设项目(STIT2017-1-11)
