摘要
Phycocyanin (PC), a natural algal protein, is reported for having anti-oxidant and antiinfl ammatory properties. We investigated its ability to attenuate lung infl ammation in mice subjected to X-ray radiation. Male C57BL/6 mice were assigned to the control, total body irradiation, PC pretreatment, and PC treatment groups. Mice in the PC pretreatment group were gavaged with 200 mg/kg PC for 7 consecutive days before irradiation, and those in the PC treatment group were gavaged with 200 mg/kg PC for 7 consecutive days after irradiation. Lungs were collected on Day 7 after irradiation exposure. Hematoxylin and eosin staining of mouse lung sections showed considerable infl ammation damage 7 days after irradiation compared with the control lung but a reduction in pathological injury in the PC treatment group. Pretreatment or treatment with PC signifi cantly decreased levels of interleukin-6 and tumor necrosis factor-α in the lung, and also increased the relative mRNA expression of superoxide dismutase and glutathione. In vivo, PC signifi cantly reduced the expression of Toll-like receptor TLR2, myeloid diff erentiation primary response Myd88, and nuclear factor NF-κB, at both the transcriptional and translation level. Taken together, these data indicated that PC attenuated lung infl ammatory damage induced by radiation by blocking the TLR2- MyD88-NF-κB signaling pathway. Therefore, PC could be a protective agent against radiation-induced infl ammatory damage in normal tissues.
Phycocyanin(PC),a natural algal protein,is reported for having anti-oxidant and antiinflammatory properties.We investigated its ability to attenuate lung inflammation in mice subjected to X-ray radiation.Male C57 BL/6 mice were assigned to the control,total body irradiation,PC pretreatment,and PC treatment groups.Mice in the PC pretreatment group were gavaged with 200 mg/kg PC for 7 consecutive days before irradiation,and those in the PC treatment group were gavaged with 200 mg/kg PC for7 consecutive days after irradiation.Lungs were collected on Day 7 after irradiation exposure.Hematoxylin and eosin staining of mouse lung sections showed considerable inflammation damage 7 days after irradiation compared with the control lung but a reduction in pathological injury in the PC treatment group.Pretreatment or treatment with PC significantly decreased levels of interleukin-6 and tumor necrosis factor-a in the lung,and also increased the relative mRNA expression of superoxide dismutase and glutathione.In vivo,PC significantly reduced the expression of Toll-like receptor TLR2,myeloid differentiation primary response Myd88,and nuclear factor NF-κB,at both the transcriptional and translation level.Taken together,these data indicated that PC attenuated lung inflammatory damage induced by radiation by blocking the TLR2-MyD88-NF-κB signaling pathway.Therefore,PC could be a protective agent against radiation-induced inflammatory damage in normal tissues.
基金
Supported by the National Key Research and Development Program of China(No.2018YFD0901102)
