摘要
目的:研究环境因子对铁皮石斛生长发育及药效成分含量的影响,探索其高产优质的人工栽培条件。方法:通过室内模拟正交试验,设置了不同光照、温度、湿度、营养水平的生长条件,培养4个月后,采用叶绿素荧光仪、比色法等方法测量了铁皮石斛的生物量、形态特征、叶绿素荧光参数及多糖、黄酮和生物碱的含量等指标。结果:在本次试验参数范围内,光照是影响生物量、多糖和叶绿素含量的首要因子,高光照(21 600 Lx)有利于生物量的积累,而低光照(2 400 Lx)则会增加叶绿素和多糖含量。空气湿度是影响茎粗、分蘖数、ETRmax、Fv/Fm和生物碱、黄酮含量的重要因子,中等水平湿度(昼/夜湿度80%/65%)有利于以上形态指标和叶绿素荧光参数,而低湿度(昼/夜湿度70%/55%)则有利于生物碱和黄酮的积累。温度是影响株高的主要因子,高温(昼/夜温度28℃/13℃)有利于株高的生长。此外,营养成分虽然显著影响了大部分的指标参数,但均不是最主要影响因子。结论:综合产量和品质分析,昼/夜温度28℃/13℃、昼/夜湿度80%/65%为铁皮石斛适宜的生长条件,但21 600 Lx高光照有利于其提高生物量,而2 400 Lx低光照则更有利于提高其多糖含量。该研究结果为人工规模化高效栽培铁皮石斛提供科学依据。
Objective:To study the effects of environmental factors on the growth and development and effective component contents of Dendrobium officinale,and to explore the aritificial cultivation conditions of high quality and high yield.Methods:The growth conditions of different light,temperature,humidity and nutrition levels were set through the simulated orthogonal experiment in the laboratory.After four months of culture,the biomass,morphological character,hlorophyll fluorescence parameters and the contents of polysaccharides,flavonoids and alkaloids were detected by chlorophyll fluoromet,colorimetry,etc.Results:Within the parameters of this experiment,light intensity was the primary factor affecting biomass,polysaccharide and chlorophyll contents,the high intensity light(21 600 Lx)was beneficial to the accumulation of biomass,low intensity light(2 400 Lx) increased chlorophyll and polysaccharide contents.The air humidity was the primary factor affecting stem diameter,tiller number,ETRmax,Fv/Fm and the contents of alkaloids and flavonoids,and the middle humidity(day/night humidity of 80%/65%)was beneficial to the above morphological indexes and chlorophyll fluorescence parameters,low humidity(day/night humidity of 70%/55%)was associated with the accumulation of alkaloids and flavonoids.Temperature was the main factor affecting plant height.The high temperature(day/night temperature of 28 ℃/13 ℃)promoted the growth of plant height.Moreover,nutrients had significant effects on most indicator parameters,but it was not the most important factor.Conclusion:Comprehensive yield and quality analysis,temperature(day/night temperature of 28 ℃/13 ℃)and relative humidity(day/night humidity of 80%/65%)are suitable growth conditions of Dendrobium officinale.However,the high intensity light(21 600 Lx)is beneficial to biomass increase,and the low intensity light(2 400 Lx)is more beneficial to improve polysaccharide content.The study can provide a scientific basis for the cultivation of Dendrobium officinale on artificial scale and efficiency.
作者
唐丽
王朝勇
龙华
李菁
陈功锡
周强
TANG Li;WANG Chao-yong;LONG Hua;LI Jing;CHEN Gong-xi;ZHOU Qiang(School of Biological Resources and Environmental Sciences,Jishou University/Key Laboratory of Plant Resources Protection and Utilization,College of Hunan Province, Jishou 416000,China;College of Agriculture and Forestry Science and Technology,Hunan Ap plied Technology University,Changde 415000,China)
出处
《中药材》
CAS
北大核心
2019年第2期251-255,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金青年项目(31300337)
广东省科技厅农业攻关项目(2013B020302003)
湖南省重点学科建设项目(JSU0713Z21)
植物资源保护与利用湖南省高校重点实验室开放项目(JSK2017YX004)
喀斯特草地生态系统湖南省研究生培养创新基地开放项目(201402B)
关键词
铁皮石斛
环境因子
药效成分
生长发育
正交试验
Dendrobium officinale Kimura et Migo
Environmental factors
Effective components
Growth and development
Orthogonal test
