摘要
该研究从不同的罗非鱼养殖区域、养殖模式、养殖环境中分别采集样品,经前期处理后得到262株待测菌株。以无乳链球菌为指示菌株,利用琼脂扩散法筛选拮抗菌。通过安全性试验对初筛菌株进行复筛,同时进行16SrDNA分子鉴定。结果显示,初筛得到29株拮抗菌,其中6株对无乳链球菌安全,分属于芽胞杆菌属、假单胞菌属、肠杆菌属3个属。
The study collected samples from different tilapia culture areas,culture models and culture environments,and obtained 262 strains after pretreatment.Streptococcus agalactiae was used as an indicator strain to screen antagonistic strain by agar diffusion method.The primarily screened strain was rescreened through a safety test,and the 16S rDNA sequence molecule was simultaneously identified.The results showed that,among 29 primarily screened strains of antagonistic bacteria,6 strains were safe to Streptococcus agalactiae,belonging to the genus Bacillus,Pseudomonas,and Enterobacter.
作者
赵光军
朱海
佟延南
韩丽娜
李志鸿
申志新
李芳远
ZHAO Guang-jun;ZHU Hai;TONG Yan-nan;HAN Li-na;LI Zhi-hong;SHEN Zhi-xin;LI Fang-yuan(Hainan Academy of Ocean and Fisheries,Haikou Hainan 571126)
出处
《现代农业科技》
2019年第14期210-211,216,共3页
Modern Agricultural Science and Technology
基金
海南省自然科学基金面上项目(317199)
2018年度海南省科研院所技术开发专项“淡水池塘养殖用水排放对自然水域环境的影响评价”
关键词
罗非鱼
养殖环境
益生菌
筛选
tilapia
culture environment
probiotics
screening
