摘要
本文建立了一种UPLC-MS/MS检测、内标法定量测定苹果、哈密瓜、青菜和白菜四种果蔬中草铵膦的快速方法.试样经水提取,C18小柱净化,9-芴甲基氯甲酸酯(FMOC-C1)衍生.用C18色谱柱分离,以0.1%甲酸水溶液和乙腈作为流动相进行梯度洗脱.质谱条件是正离子模式电离,多反应监测,内标法定量.结果表明,草铵膦在10~500 ng/mL内呈线性相关,R2为0.999,加标回收率为85.5% ~97.4%,相对标准偏差为3.0% ~8.2%,检出限为20μg/kg.本方法成本低廉,分析灵敏,定性定量准确,可以满足草铵膦残留量的测定.
A rapid method of determination of glufosinate in apple,cantaloupe,green vegetables and Chinese Cabbage by UPLC-MS/MS and internal standard method was established.The sample was extracted by water,purified by C18 column and derived by 9-fluorene methyl chloroformate (FMOC-C1).C18 column was used for separation and gradient elution with 0.1% formic acid and acetonitrile as mobile phase.Mass spectrometry conditions are positive ion mode ionization,multi-reaction monitoring,and internal standard quantification.The results showed that glufosinate showed a linear correlation in the range of 10~1000 ng/mL,R2 was 0.999,the recovery was 85.5%~97.4%,the relative standard deviation was 3.0%~8.2%,and the detection limit was 20 μg/kg.The method has the advantages of low cost,sensitive analysis,accurate qualitative and quantitative analysis,and can be used for the determination of glufosinat residues.
作者
施雅
Shi Ya(Shanghai Bino Testing Service Co.,Ltd.,Shanghai200436,China)
出处
《山东化工》
CAS
2019年第13期100-102,共3页
Shandong Chemical Industry
