摘要
建立了一种基于核酸适配体识别-纳米金显色的盐酸克伦特罗可视化检测方法。通过合成纳米金、适配体、适配体互补链以及适配体-纳米金探针和互补链-纳米金探针,利用纳米金的变色效应,构建了盐酸克伦特罗的简单、快速、高灵敏度检测方法。当待测物中含有目标物时,适配体与目标物结合,纳米金呈现游离状态,在一定的盐浓度下,纳米颗粒发生聚集,纳米金颜色发生变化;当待测物中不含目标物时,适配体与适配体互补链互补杂交,形成稳定的网络结构,溶液颜色不发生变化。分别对适配体、互补链与纳米金连接的陈化盐浓度、适配体与互补链浓度、显色体系盐浓度等参数进行了优化。在优化的条件下,盐酸克伦特罗在1~1000 ng/mL浓度范围内,呈现良好的线性关系,回归方程为y=0.023 x+0.362(R2=0.991),最低检测限为1 ng/mL。对猪肝实际样品的加标回收率为83.5%~101.8%。该方法简单、准确、可靠,可用于实际样品的检测分析。
To detect clenbuterol(CL),aβ-Agonist,a visual and rapid method based on aptamer and gold nanoparticles(AuNPs)was established in this study.The aptamer(DNA1)conjugating with clenbuterol,and its complementary DNA(DNA2)were designed and synthetized,respectively.The aptamer-AuNPs probe(probe1)and aptamer complementary DNA-AuNPs probe(probe2)were also synthetized in present study.In the presence of CL,aptamer bound to the target,AuNPs maintained a free state,however,these AuNPs began to aggregate and change color from red to purple in a high concentration of salt.In the absence of CL,aptamer and its complementary DNA formed a stable network,keeping red color.Some parameters,such as the concentration of aptamer,aptamer complementary DNA and salt were optimized,respectively.The results showed that in the optimum conditions,clenbuterol had a good linear relationship ranging from 1 to 1000 ng/mL,with correlation coefficients(R2)0.991.The visual detection limit was 1 ng/g,and the recoveries at three spike levels ranged from 83.5%to 101.8%.This newly developed method was simple,sensitive,and reliable.It was suitable to detect clenbuterol in actual samples.
作者
张娜
韦丽婷
李向丽
刘妍
谭贵良
王周平
郭艳峰
ZHANG Na;WEI Li-ting;LI Xiang-li;LIU Yan;TAN Gui-liang;WANG Zhou-ping;GUO Yan-feng(Department of Biomedicine,Zhongshan Torch Polytechnic,Zhongshan 528436,China;Food College Jiangnan University,Wuxi 214122,China;Zhongshan College,University Electronic Science Technology of China,Zhongshan 528402,China;Zhongshan Institute for Food and Drug Control,Zhongshan 528437,China)
出处
《现代食品科技》
EI
CAS
北大核心
2019年第7期301-306,29,共7页
Modern Food Science and Technology
基金
广东省食药局科技计划项目(2015ZX07)
中山市科技计划项目(2015B2353)
